Department of Biomedical, Experimental, and Clinical Sciences "Mario Serio", University of Florence, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
Mol Reprod Dev. 2021 Feb;88(2):175-184. doi: 10.1002/mrd.23447. Epub 2020 Dec 18.
Research has been focused on determining the follicular microenviroment produced by the theca and granulosa cells since the molecular characterisation of this body fluid could lead to the understanding of several fertility problems. Oxidative stress may be one of the factors involved in female infertility since it plays a key role in the modulation of oocyte maturation and finally pregnancy. An increase in oxidative stress is correlated with inflammation and intense research was developed to understand the interaction between inflammation and adiponectin, based on the fact that many adipokines are inflammation related proteins linked to reactive oxygen species production. The aim of this study is to investigate the correlation between total adiponectin levels and oxidative stress amount in the serum and follicular fluid (FF) of women who undergone in vitro fertilization. Moreover we verified the expression of adiponectin in granulosa and cumulus cells. To clarify the predictive value of steroid hormones in human assisted reproduction, twelve steroid hormones in FF and serum, were quantified in a single run liquid chromatography/mass spectrometry, by using a multiple reaction monitoring mode and we related the serum and follicular fluids adiponectin levels with the concentration of the investigated steroid hormones.
研究一直集中在确定由卵泡膜细胞和颗粒细胞产生的卵泡微环境，因为这种体液的分子特征可能导致对几种生育问题的理解。氧化应激可能是女性不孕的因素之一，因为它在卵母细胞成熟的调节中起着关键作用，最终导致怀孕。氧化应激的增加与炎症有关，并且已经进行了大量的研究来理解炎症和脂联素之间的相互作用，基于许多脂肪因子是与活性氧物质产生相关的炎症相关蛋白这一事实。本研究旨在调查在接受体外受精的女性的血清和卵泡液（FF）中总脂联素水平与氧化应激量之间的相关性。此外，我们还验证了脂联素在颗粒细胞和卵丘细胞中的表达。为了阐明类固醇激素在人类辅助生殖中的预测价值，我们在单次运行液相色谱/质谱法中对 FF 和血清中的 12 种类固醇激素进行了定量，使用多重反应监测模式，并将血清和卵泡液中的脂联素水平与所研究的类固醇激素的浓度相关联。
