脉冲交错式 MINFLUX。
Pulsed Interleaved MINFLUX.
机构信息
Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad Autónoma de Buenos Aires, Argentina.
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Güiraldes 2620, C1428EHA, Ciudad Autónoma de Buenos Aires, Argentina.
出版信息
Nano Lett. 2021 Jan 13;21(1):840-846. doi: 10.1021/acs.nanolett.0c04600. Epub 2020 Dec 18.
We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.
我们介绍了 p-MINFLUX，这是一种新实现的高度光子效率的单分子定位方法，具有简化的实验设置和额外的荧光寿命信息。与原始的 MINFLUX 实现相比，p-MINFLUX 使用交错的激光脉冲以最大重复率传递环形激发焦点。使用静态和动态 DNA 折纸模型系统，我们分别展示了 p-MINFLUX 用于单分子定位纳米显微镜和跟踪的性能。p-MINFLUX 提供 1-2nm 的定位精度，需要 2000-1000 个光子计数。此外，p-MINFLUX 还可以获得荧光寿命信息，从而实现多路复用和超分辨寿命成像。p-MINFLUX 应该有助于释放创新的单分子定位方案的全部潜力。
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