Division of Plastic and Reconstruction Surgery, Department of Surgery, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chia-Yi City, Taiwan.
Department of Food Nutrition and Health Biotechnology, College of Medical and Health Science, Asia University, Taichung City, Taiwan.
Environ Toxicol. 2021 May;36(5):729-736. doi: 10.1002/tox.23075. Epub 2020 Dec 18.
Oxidative stress is a major cause of aging related skin injuries. Hydrogen peroxide related ROS accumulation triggers increase in matrix metalloproteinases and elevated collagen degradation, which is a characteristic of skin aging. In this study, we investigated the protective effect of Poria cocos, used widely in the treatment of inflammatory diseases, against H O induced oxidative stress. The aqueous extract of dried P. cocos was obtained by heating 10 g in 500 ml of distilled water. The mixture was evaporated up to 400 ml and the remaining 100 ml was filtered through muslin cloth repeatedly to obtain a clear aqueous extract of the P. cocos. Hs68 human dermal fibroblast cells were challenged with 100 μM of H O for 24 h. Following H O challenge, the cells were treated with increasing concentration of P. cocos extract (100-400 μg/ml) for 24 h. P. cocos extract hindered the H O induced cell death significantly that was correlated with reduction in ROS accumulation. Western blot analysis show that P. cocos extract suppressed the expression of metallomatrix proteinases, inflammatory markers and skin aging markers, but increased TGF-β1 levels and antioxidant related proteins. These data suggest that P. cocos is effective in attenuating oxidative stress associated skin aging effects and may be a potential agent in cosmetics products.
氧化应激是导致与衰老相关的皮肤损伤的主要原因。过氧化氢相关的 ROS 积累会引发基质金属蛋白酶的增加和胶原蛋白降解的升高，这是皮肤衰老的特征。在这项研究中，我们研究了广泛用于治疗炎症性疾病的茯苓对 H2O2 诱导的氧化应激的保护作用。将 10 g 干茯苓加热在 500 ml 蒸馏水中获得茯苓的水提物。将混合物蒸发至 400 ml，并用粗棉布反复过滤剩余的 100 ml，以获得茯苓的澄清水溶液提取物。用 100 μM H2O2 处理人真皮成纤维细胞（Hs68）24 h。在 H2O2 处理后，用递增浓度的茯苓提取物（100-400 μg/ml）处理细胞 24 h。茯苓提取物显著抑制 H2O2 诱导的细胞死亡，这与 ROS 积累的减少相关。Western blot 分析表明，茯苓提取物抑制了基质金属蛋白酶、炎症标志物和皮肤衰老标志物的表达，而增加了 TGF-β1 水平和抗氧化相关蛋白。这些数据表明，茯苓可有效减轻与氧化应激相关的皮肤衰老作用，可能是化妆品产品中的一种潜在有效成分。
