Dewitte J D, Berthou F, Dreano Y, Floch H H
Laboratoire de Biochimie: Unité de Chromatographie, Faculté de Médecine de Brest, France.
Biomed Chromatogr. 1987;2(5):183-8. doi: 10.1002/bmc.1130020502.
In order to improve the urinary neopterin measurement, the reversed-phase HPLC method has been reevaluated. The parameters which influence the chromatographic behavior of 12 pteridines were studied: nature of buffer, pH, ionic strength, addition of organic modifier to the mobile phase. Accordingly, an isocratic HPLC method is described which offers a good compromise between specificity and analysis time. This method is well-suited to automation in routine clinical laboratory use. Using this HPLC method, urinary neopterin related to creatinine was determined in lung diseases (neoplasm, sarcoïdosis and bronchial asthma) and in kidney allografts. This method was shown to be useful in the diagnosis and in the monitoring of treatment of rejection episodes.
为了改进尿新蝶呤的测定方法，对反相高效液相色谱法进行了重新评估。研究了影响12种蝶啶色谱行为的参数：缓冲液性质、pH值、离子强度、向流动相中添加有机改性剂。据此，描述了一种等度高效液相色谱法，该方法在特异性和分析时间之间取得了良好的平衡。该方法非常适合常规临床实验室使用的自动化操作。使用这种高效液相色谱法，测定了肺部疾病（肿瘤、结节病和支气管哮喘）及肾移植受者尿液中与肌酐相关的新蝶呤。结果表明该方法在排斥反应的诊断和治疗监测中具有实用价值。
