Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA.
Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA.
Stem Cell Reports. 2021 Jan 12;16(1):212-223. doi: 10.1016/j.stemcr.2020.11.011. Epub 2020 Dec 17.
Most of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock.
我们目前关于早期谱系特化和胚胎衍生干细胞的大部分知识来自于啮齿动物模型的研究。然而，我们对这些发育过程的理解在非啮齿动物物种中仍然存在关键的差距。在这里，我们报告了猪胚外内胚层（pXEN）细胞的详细特征，这些细胞可以从囊胚的原始内胚层（PrE）中可靠且可重复地产生。高度可扩增的 pXEN 细胞表达典型的 PrE 标记物，并在转录上类似于啮齿动物的 XENs。当将 pXEN 细胞注入宿主囊胚时，它们既可以贡献胚外组织，包括内脏卵黄囊，也可以贡献胚胎肠道，并且当用作体细胞核移植（SCNT）的核供体时，可以产生活后代。pXEN 细胞系为研究谱系分离提供了一个新的模型，同时也为家畜的基因组编辑提供了一个来源。
