Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia de ambientes Aquáticos Continentais, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil.
Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Ciências Fisiológicas, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Apr;242:108955. doi: 10.1016/j.cbpc.2020.108955. Epub 2020 Dec 15.
The entrance of the anti-inflammatory diclofenac in water bodies is a consequence of inappropriate use, incorrect disposal, and inefficiency of wastewater treatment plants (WWTPs) in removing this drug from sewage, among others. Effects of diclofenac on non-target aquatic organisms still need to be clarified. The objective of this work was to evaluate the toxic effects of the diclofenac on larvae and adults of Danio rerio. The LC values were 5.49 mg/L and 5.22 mg/L for the adult and larvae, respectively. A set of biochemical and genotoxic biomarkers were evaluated in fish exposed to an environmentally relevant concentration of 2 μg/L DCF and a no observed effect concentration (NOEC) of 3 mg/L diclofenac. At the NOEC, an increase in activities of glutathione-S-transferase (GST) enzyme and an increase in ATP binding cassette (ABC) transporters in gills of adult fish was observed; also, an increase in lipoperoxidation (LPO) was seen in the gills of adults and whole larvae. These results indicate that diclofenac activates the fish detoxification processes and may affect fish health.
抗炎药双氯芬酸进入水体是由于不当使用、处理不当以及废水处理厂（WWTP）未能有效去除污水中的药物等原因造成的。双氯芬酸对非目标水生生物的影响仍需进一步阐明。本研究旨在评估双氯芬酸对斑马鱼幼虫和成鱼的毒性作用。双氯芬酸对成鱼和幼虫的半数致死浓度（LC）值分别为 5.49 mg/L 和 5.22 mg/L。在暴露于环境相关浓度 2 μg/L DCF 和无观察到效应浓度（NOEC）3 mg/L 双氯芬酸的条件下，评估了一组生化和遗传毒性生物标志物。在 NOEC 下，观察到成鱼鳃中谷胱甘肽-S-转移酶（GST）酶活性增加和三磷酸腺苷结合盒（ABC）转运蛋白增加，同时成鱼和整个幼虫的鳃中脂质过氧化（LPO）增加。这些结果表明，双氯芬酸激活了鱼类的解毒过程，可能会影响鱼类健康。
