Cyanobacterial Biotechnology Lab, Jamia Millia Islamia University, New Delhi 110025, India.
School of Biotechnology, KIIT University, Bhubaneswar, India.
Bioorg Chem. 2021 Feb;107:104535. doi: 10.1016/j.bioorg.2020.104535. Epub 2020 Dec 11.
With the rapid development of nanotechnology, much has been anticipated with silver nanoparticles (AgNPs) due to their extensive industrial and commercial applications. However, it has raised concerns over environmental safety and human health effects. In this study, AgNPs were bio-fabricated using aqueous extract of Microchaete and their medical applications like antioxidant, anti-proliferative, and apoptosis were done. The biosynthesis of AgNPs was continuously followed by UV-vis spectrophotometric analysis. The physiochemical properties like shape, size, crystallinity, and polydispersity of the nanoparticles were determined by Scanning Electron Microscopy (SEM) along with EDX, Transmission Electron Microscope (TEM), Atomic Force Microscope (AFM), dynamic light scattering (DLS), and X-Ray Diffraction (XRD). Biosynthesized 7.0 nm sized AgNPs with the crystalline structure (crystalline size 4.8 nm) having a hydrodynamic diameter of 38.74 ± 2.6 nm was achieved due to the involvement of reducing agents present in the cyanobacterial extract. The IC values of the AgNPs were evaluated as 75 µg/ml and 79.41 µg/ml with HepG2 and MCF-7 cell lines. Different in-vitro cellular assays investigated in the present study exhibited antioxidant, anti-proliferative, and apoptotic activities. Probably delayed apoptosis in HepG2 and MCF-7 is due to better antioxidant activities of Microchaete based AgNPs.
随着纳米技术的快速发展，由于银纳米粒子（AgNPs）具有广泛的工业和商业应用，因此人们对其寄予厚望。然而，这引发了人们对环境安全和人类健康影响的关注。在这项研究中，使用 Microchaete 的水提物生物制造了 AgNPs，并对其抗氧化、抗增殖和细胞凋亡等医学应用进行了研究。AgNPs 的生物合成通过紫外可见分光光度法分析进行了连续监测。通过扫描电子显微镜（SEM）以及能量色散 X 射线能谱分析（EDX）、透射电子显微镜（TEM）、原子力显微镜（AFM）、动态光散射（DLS）和 X 射线衍射（XRD）等方法，确定了纳米粒子的物理化学性质，如形状、尺寸、结晶度和多分散性。由于蓝细菌提取物中存在的还原剂的参与，成功地合成了具有 7.0nm 尺寸和 4.8nm 结晶尺寸（晶粒度）的结晶结构的 AgNPs，其水动力直径为 38.74±2.6nm。AgNPs 的 IC 值在 HepG2 和 MCF-7 细胞系中分别评估为 75μg/ml 和 79.41μg/ml。本研究中进行的不同体外细胞试验显示出抗氧化、抗增殖和细胞凋亡活性。可能是由于基于 Microchaete 的 AgNPs 具有更好的抗氧化活性，导致 HepG2 和 MCF-7 中的细胞凋亡延迟。
