Amity Institute of Biotechnology, Amity University Madhya Pradesh, Gwalior, 474005, India.
School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
Curr Microbiol. 2024 Nov 30;82(1):23. doi: 10.1007/s00284-024-03983-4.
Recent developments in nanotechnology have revealed the significance of bimetallic nanoparticles for various applications. This study reveals the facile green synthesis of Ag-Co bimetallic nanoparticles using eugenol. A comparative analysis of Eugenol-loaded Ag-Co and unloaded Ag-Co bimetallic nanoparticle (BNPs) was done to investigate their antibacterial and antioxidant activity using flow cytometry. The biosynthesized Eugenol-loaded and unloaded BNPs were evaluated for antibacterial activity against Escherichia coli. The antioxidant activity was analyzed by using a DPPH scavenging activity assay and flow cytometry. UV-Vis spectroscopic analysis of synthesized eugenol-loaded and unloaded BNPs showed absorbance at 257 nm and 240 nm, respectively. FTIR analysis showed the peak range in the 500-4000 cm- corresponds to different functional groups. Zeta potential confirms the formation of stable and uniformly synthesized BNPs. EDX and SEM analysis confirm the elemental compositions and the size of the BNPs, respectively. Flow cytometric analysis revealed the live-dead parameters of Escherichia coli, when exposed to different concentrations of Eugenol-loaded Ag-Co (Eu@Ag-Co) and Unloaded Ag-Co BNPs. In addition, comparative MIC and MBC values of eugenol-loaded and unloaded BNPs were obtained for Escherichia coli. The Antioxidant activity revealed the comparative higher significant DPPH scavenging activity of Eu@Ag-Co. Moreover, the flow cytometric analysis confirmed the higher antibacterial efficacy of Eu@Ag-Co over unloaded Ag-Co BNPs by showing a higher percentage of dead cells. The study determined the enhanced antibacterial and antioxidant activity of nanoparticles by Eugenol loading and advocated it to be a better therapeutic approach. This study encourages the use of biosynthesized loaded-phytochemicals BNPs over unloaded BNPs.
近年来，纳米技术的发展揭示了双金属纳米粒子在各种应用中的重要性。本研究利用丁香酚揭示了 Ag-Co 双金属纳米粒子的简便绿色合成。通过流式细胞术对负载丁香酚的 Ag-Co 和未负载 Ag-Co 双金属纳米粒子（BNPs）进行了比较分析，以研究它们的抗菌和抗氧化活性。合成的负载丁香酚和未负载 BNPs 用于测试其对大肠杆菌的抗菌活性。通过 DPPH 清除活性测定和流式细胞术分析了抗氧化活性。合成的负载丁香酚和未负载 BNPs 的紫外可见光谱分析显示，在 257nm 和 240nm 处分别有吸收峰。FTIR 分析表明，在 500-4000cm-1 范围内的峰对应于不同的官能团。Zeta 电位证实了稳定且均匀合成 BNPs 的形成。EDX 和 SEM 分析分别证实了 BNPs 的元素组成和尺寸。流式细胞术分析揭示了大肠杆菌暴露于不同浓度的负载丁香酚的 Ag-Co（Eu@Ag-Co）和未负载 Ag-Co BNPs 时的死活参数。此外，还获得了负载丁香酚和未负载 BNPs 对大肠杆菌的 MIC 和 MBC 值。抗氧化活性表明，Eu@Ag-Co 的 DPPH 清除活性更高。此外，流式细胞术分析通过显示更高比例的死细胞证实了 Eu@Ag-Co 比未负载 Ag-Co BNPs 具有更高的抗菌功效。该研究确定了负载丁香酚的纳米粒子增强了抗菌和抗氧化活性，并主张将其作为一种更好的治疗方法。本研究鼓励使用生物合成的负载植物化学物质的 BNPs 代替未负载的 BNPs。
