School of Life Science, Beijing University of Chinese Medicine, Beijing, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Prep Biochem Biotechnol. 2021;51(2):120-126. doi: 10.1080/10826068.2020.1799389. Epub 2020 Dec 20.
Ginseng () is a famous Traditional Chinese Medicine, which is widely used to treat cardiovascular disease. () is a fungus used in food and medicine fermentation, and lovastatin, its metabolite, is used extensively in the treatment of dyslipidemia. In this study, ginseng has been fermented by , and the response surface methodology (RSM) was applied to optimize fermentation parameters to obtain optimal fermentation system, with further exploring to lipid-lowering activity of - fermentation products (PM). The concentration of ginseng, temperature, and rotating speed were set as variables and the lovastatin yield was optimized by a Box-Behnken design (BBD) analyzed by RSM. The binding capacity of PM for sodium taurocholate and sodium cholate was assayed by UV spectrophotometry. The highest content of lovastatin production (85.53 μg g) was obtained at a ginseng concentration of 1.96%, temperature of 30.11 °C, and a rotating speed of 160.47 rpm. PM exhibited bile acid binding capacity, which was stronger than unfermented ginseng. The RSM can be used to optimize the fermentation system to obtain the best fermentation process. In addition, the fermentation of ginseng by can enhance the lipid-lowering effect.
人参是一种著名的中药，广泛用于治疗心血管疾病。（）是一种用于食品和药物发酵的真菌，其代谢产物洛伐他汀广泛用于治疗血脂异常。在这项研究中，用人参发酵，响应面法（RSM）被应用于优化发酵参数，以获得最佳发酵体系，并进一步探索（）发酵产物（PM）的降血脂活性。人参浓度、温度和转速被设定为变量，通过 RSM 分析的 Box-Behnken 设计（BBD）对洛伐他汀产量进行优化。通过紫外分光光度法测定 PM 对牛磺胆酸钠和胆酸钠的结合能力。在人参浓度为 1.96%、温度为 30.11°C、转速为 160.47rpm 的条件下，洛伐他汀的产量最高（85.53μg/g）。PM 表现出胆汁酸结合能力，比未发酵的人参更强。RSM 可用于优化发酵体系，以获得最佳发酵工艺。此外，用发酵可以增强人参的降血脂作用。
