Department of Laboratory Medicine, Key Laboratory of Medical Diagnostics of Ministry of Education, Chongqing Medical University, No. 1 Yi Xue Yuan Road, Chongqing, 400016, People's Republic of China.
Traditional Chinese Medicine Hospital of Chongqing, Chongqing, 400021, People's Republic of China.
Mikrochim Acta. 2020 Mar 11;187(4):216. doi: 10.1007/s00604-020-4213-z.
An integrated electrochemical immunoassay is described for the determination of circulating tumor cells (CTCs). For the first time, Ketjen black (KB), which is a superconductive carbon material, was incorporated with Au nanoparticles (AuNPs) and used to modify the surface of gold electrodes. A cocktail of anti-epithelial cell adhesion molecules (EpCAM) and anti-vimentin antibodies was chosen to capture the CTCs. Palladium-iridium-boron-phosphorus alloy-modified mesoporous nanospheres (PdIrBPMNS) served as a catalytic tag to amplify the current signal. Glycine-HCl (Gly-HCl) was used as an antibody eluent to release and collect the captured CTCs from the electrodes for further clinical research without compromising cell viability. The response of the method increased linearly from 10 to 1 × 10 cells mL CTCs, while the detection limit was calculated to be as low as 2 cells mL. This method was successfully used to determine CTCs in spiked blood samples and demonstrated good recovery. Graphical abstractKetjen black/AuNPs was incorporated in the electrochemical platform to enhance the electron transfer ability of the electrode surface. PdIrBP mesoporous nanospheres were used to amplify DPV signal in this assay. The introduction of Gly-HCl realized nondestructive recovery of circulating tumor cells.
一种用于检测循环肿瘤细胞（CTC）的集成电化学免疫分析方法。首次将超导体碳材料凯夫拉（Ketjen black，KB）与金纳米粒子（AuNPs）结合，用于修饰金电极表面。选择了抗上皮细胞黏附分子（EpCAM）和抗波形蛋白抗体的混合物来捕获 CTC。钯-铱-硼-磷合金修饰的介孔纳米球（PdIrBPMNS）作为催化标签，放大电流信号。甘氨酸-盐酸（Gly-HCl）用作抗体洗脱液，从电极上释放和收集捕获的 CTC，用于进一步的临床研究，而不会影响细胞活力。该方法的响应从 10 个到 1×10 个细胞 mL 的 CTCs 呈线性增加，而检测限计算低至 2 个细胞 mL。该方法成功用于检测加标血样中的 CTCs，并表现出良好的回收率。
