Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China.
Traditional Chinese Medicine Hospital of Chongqing, Chongqing 400021, PR China.
Biosens Bioelectron. 2018 May 1;104:72-78. doi: 10.1016/j.bios.2018.01.001. Epub 2018 Jan 4.
Circulating tumor cells (CTCs), as the cellular origin of metastasis, are cancer cells that break away from a primary tumor and circulate in the peripheral blood. And they provide a wealth of information about tumor phenotype. Here, this work reported a novel ultrasensitive immunoassay protocol for the detection of CTCs by using Pt@Ag nanoflowers (Pt@AgNFs) and AuNPs/Acetylene black (AuNPs/AB) nanomaterial. In the established approach, AuNPs/AB nanomaterial was used as substrate material to increase the specific surface area and enhance the conductivity of the gold electrode. Protein G was used for oriented immobilization of capture antibody, which strongly improved the capture efficiency of MCF-7 cells. The innovatively synthesized Pt@AgNFs by our group with high specific surface area and good biocompatibility were not only as the carriers of signal antibodies (Ab) but also catalyzed the reduction of HO, which effectually amplified the current signal. A linear relationship between current signals and the concentrations of CTCs was obtained in the range from 20 to 1×10 cells mL and the detection limit is as low as 3 cells mL on condition of acceptable stability and reproducibility. Furthermore, the as-proposed cytosensor showed excellent performance in the detection of CTCs in human blood samples. These results suggest that the proposed cytosensor will be a promising application for accurately quantitative detection of CTCs.
循环肿瘤细胞（CTCs）作为转移的细胞起源，是从原发性肿瘤脱离并在周围血液中循环的癌细胞。它们提供了丰富的肿瘤表型信息。在这里，本工作报道了一种新颖的超灵敏免疫分析方案，用于通过使用 Pt@Ag 纳米花（Pt@AgNFs）和 AuNPs/乙炔黑（AuNPs/AB）纳米材料来检测 CTCs。在建立的方法中，AuNPs/AB 纳米材料被用作基底材料，以增加比表面积并增强金电极的导电性。蛋白 G 用于定向固定捕获抗体，这大大提高了 MCF-7 细胞的捕获效率。我们小组创新合成的 Pt@AgNFs 具有高比表面积和良好的生物相容性，不仅作为信号抗体（Ab）的载体，而且还催化了 HO 的还原，有效地放大了电流信号。在可接受的稳定性和重现性条件下，电流信号与 CTCs 浓度在 20 到 1×10 个细胞 mL 之间呈线性关系，检测限低至 3 个细胞 mL。此外，所提出的细胞传感器在人血样中 CTCs 的检测中表现出优异的性能。这些结果表明，所提出的细胞传感器将是用于 CTCs 准确定量检测的有前途的应用。
