Anders Malte, Anders Björn, Kreuzer Matthias, Zinn Sebastian, Walter Carmen
Institute of Clinical Pharmacology, Goethe University, Frankfurt am Main, Germany.
Department for Human Experimental Pain Models, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Translational Medicine and Pharmacology (TMP), Frankfurt am Main, Germany.
Front Hum Neurosci. 2020 Dec 2;14:559969. doi: 10.3389/fnhum.2020.559969. eCollection 2020.
Evoked potentials in the amplitude-time spectrum of the electroencephalogram are commonly used to assess the extent of brain responses to stimulation with noxious contact heat. The magnitude of the - and -waves are used as a semi-objective measure of the response to the painful stimulus: the higher the magnitude, the more painful the stimulus has been perceived. The strength of the --wave response is also largely dependent on the chosen reference electrode site. The goal of this study was to examine which reference technique excels both in practical and theoretical terms when analyzing noxious contact heat evoked potentials (CHEPS) in the amplitude-time spectrum. We recruited 21 subjects (10 male, 11 female, mean age of 55.79 years). We applied seven noxious contact heat stimuli using two temperatures, 51°C, and 54°C, to each subject. During EEG analysis, we aimed to identify the referencing technique which produces the highest -wave and -wave amplitudes with as little artifactual influence as possible. For this purpose, we applied the following six referencing techniques: mathematically linked A1/A2 (earlobes), average reference, REST, AFz, Pz, and mathematically linked PO7/PO8. We evaluated how these techniques impact the - amplitudes of CHEPS based on our data from healthy subjects. Considering all factors, we found that mathematically linked earlobes to be the ideal referencing site to use when displaying and evaluating CHEPS in the amplitude-time spectrum.
脑电图幅度-时间谱中的诱发电位通常用于评估大脑对有害接触热刺激的反应程度。δ波和θ波的幅度被用作对疼痛刺激反应的半客观度量：幅度越高，所感知到的刺激越疼痛。δ波反应的强度在很大程度上也取决于所选的参考电极位置。本研究的目的是检验在分析幅度-时间谱中的有害接触热诱发电位（CHEPS）时，哪种参考技术在实际和理论方面都表现出色。我们招募了21名受试者（10名男性，11名女性，平均年龄55.79岁）。我们对每个受试者应用了两种温度（51°C和54°C）的七种有害接触热刺激。在脑电图分析过程中，我们旨在确定能产生最高δ波和θ波幅度且尽可能减少伪迹影响的参考技术。为此，我们应用了以下六种参考技术：数学关联的A1/A2（耳垂）、平均参考、REST、AFz、Pz和数学关联的PO7/PO8。我们根据来自健康受试者的数据评估了这些技术如何影响CHEPS的δ波幅度。综合考虑所有因素，我们发现数学关联的耳垂是在幅度-时间谱中显示和评估CHEPS时使用的理想参考部位。
