脱嘌呤作用会在SOS诱导的细胞中引发突变。
Depurination causes mutations in SOS-induced cells.
作者信息
Schaaper R M, Loeb L A
出版信息
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1773-7. doi: 10.1073/pnas.78.3.1773.
Abstract
Introduction of apurinic sites into phi X174 am3 DNA leads to loss of biological activity when measured in a transfection assay. For single-stranded DNA, approximately one apurinic site constitutes a lethal hit; for double-stranded (RFI) DNA, approximately 3.5 hits per strand are lethal. When the reversion frequency of am3 DNA is measured, no increase due to depurination is observed above the background level. However, a large increase in reversion frequency is observed when the same DNA is assayed by using spheroplasts derived from bacteria previously exposed to UV light. The results suggest that apurinic sites are impediments to a replicating DNA polymerase; however, nucleotides can be incorporated opposite these sites under SOS-induced conditions. We estimate the frequency of mutagenesis per apurinic site to be less than 1 in 1400 in normal spheroplasts and 1 in 100 in SOS-induced spheroplasts.
摘要
在转染试验中检测时，将无嘌呤位点引入噬菌体φX174 am3 DNA会导致生物活性丧失。对于单链DNA，大约一个无嘌呤位点就构成一个致死性损伤；对于双链（RFI）DNA，每条链大约3.5个损伤是致死性的。当测量am3 DNA的回复突变频率时，在背景水平之上未观察到由于脱嘌呤导致的增加。然而，当使用先前暴露于紫外线的细菌来源的原生质球检测相同DNA时，观察到回复突变频率大幅增加。结果表明，无嘌呤位点是复制性DNA聚合酶的障碍；然而，在SOS诱导的条件下，核苷酸可以掺入这些位点相对的位置。我们估计，在正常原生质球中，每个无嘌呤位点的诱变频率小于1/1400，在SOS诱导的原生质球中为1/100。
相似文献
1
Depurination causes mutations in SOS-induced cells.脱嘌呤作用会在SOS诱导的细胞中引发突变。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1773-7. doi: 10.1073/pnas.78.3.1773.
2
Infidelity of DNA synthesis associated with bypass of apurinic sites.与无嘌呤位点绕过相关的DNA合成错误
Proc Natl Acad Sci U S A. 1983 Jan;80(2):487-91. doi: 10.1073/pnas.80.2.487.
3
Depurination of DNA as a possible mutagenic pathway for cells.DNA的脱嘌呤作用作为细胞一种可能的诱变途径。
Basic Life Sci. 1982;20:199-211. doi: 10.1007/978-1-4613-3476-7_12.
4
Chemically altered apurinic sites in phi X174 DNA give increased mutagenesis in SOS-induced E. coli.
Mutat Res. 1993 Aug;288(2):207-14. doi: 10.1016/0027-5107(93)90086-u.
5
Mutagenesis resulting from depurination is an SOS process.由脱嘌呤引起的诱变是一种SOS过程。
Mutat Res. 1982 Nov;106(1):1-9. doi: 10.1016/0027-5107(82)90186-5.
6
Mutational specificity of depurination.脱嘌呤的突变特异性
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1494-8. doi: 10.1073/pnas.81.5.1494.
7
Role of depurination in mutagenesis by chemical carcinogens.
Cancer Res. 1982 Sep;42(9):3480-5.
8
Mutagenesis in vitro by depurination of phiX174 dna.通过φX174 DNA脱嘌呤作用进行的体外诱变
Nature. 1981 May 28;291(5813):349-51. doi: 10.1038/291349a0.
9
Some characteristics of apurinic sites in single- and double-stranded biologically active phi X174 DNA.单链和双链具有生物活性的φX174噬菌体DNA中无嘌呤位点的一些特征。
Nucleic Acids Res. 1981 Dec 11;9(23):6591-9. doi: 10.1093/nar/9.23.6591.
10
Depurination-induced infidelity of deoxyribonucleic acid synthesis with purified deoxyribonucleic acid replication proteins in vitro.体外利用纯化的脱氧核糖核酸复制蛋白研究脱嘌呤诱导的脱氧核糖核酸合成错误率
Biochemistry. 1983 May 10;22(10):2378-84. doi: 10.1021/bi00279a012.
引用本文的文献
1
Bracken Fern Carcinogen, Ptaquiloside, Forms a Guanine -Adduct in DNA.蕨菜致癌物原蕨苷在DNA中形成鸟嘌呤加合物。
J Agric Food Chem. 2025 Jan 15;73(2):1053-1061. doi: 10.1021/acs.jafc.4c07187. Epub 2025 Jan 7.
2
DNA damage alters binding conformations of E. coli single-stranded DNA-binding protein.DNA 损伤改变大肠杆菌单链 DNA 结合蛋白的结合构象。
Biophys J. 2023 Oct 3;122(19):3950-3958. doi: 10.1016/j.bpj.2023.08.018. Epub 2023 Aug 24.
3
Understanding the importance of low-molecular weight (ethylene oxide- and propylene oxide-induced) DNA adducts and mutations in risk assessment: Insights from 15 years of research and collaborative discussions.了解低分子量（环氧乙烷和环氧丙烷诱导的）DNA加合物及突变在风险评估中的重要性：来自15年研究与合作讨论的见解
Environ Mol Mutagen. 2019 Mar;60(2):100-121. doi: 10.1002/em.22248. Epub 2018 Dec 10.
4
Non-enzymatic depurination of nucleic acids: factors and mechanisms.核酸的非酶促脱嘌呤作用：影响因素及机制
PLoS One. 2014 Dec 29;9(12):e115950. doi: 10.1371/journal.pone.0115950. eCollection 2014.
5
Mutational consequences of dNTP pool imbalances in E. coli.大肠杆菌中 dNTP 池失衡的突变后果。
DNA Repair (Amst). 2013 Jan 1;12(1):73-9. doi: 10.1016/j.dnarep.2012.10.011. Epub 2012 Dec 6.
6
On the formation and properties of interstrand DNA-DNA cross-links forged by reaction of an abasic site with the opposing guanine residue of 5'-CAp sequences in duplex DNA.在双链 DNA 中，碱基缺失位点与 5'-CAp 序列中对面的鸟嘌呤残基反应形成的链间 DNA-DNA 交联的形成和性质。
J Am Chem Soc. 2013 Jan 23;135(3):1015-25. doi: 10.1021/ja308119q. Epub 2013 Jan 11.
7
Primer extension mutagenesis powered by selective rolling circle amplification.引物延伸诱变技术通过选择性滚环扩增驱动。
PLoS One. 2012;7(2):e31817. doi: 10.1371/journal.pone.0031817. Epub 2012 Feb 15.
8
Chemistry and structural biology of DNA damage and biological consequences.DNA 损伤及其生物学后果的化学与结构生物学。
Chem Biodivers. 2011 Sep;8(9):1571-615. doi: 10.1002/cbdv.201100033.
9
Chinese hamster apurinic/apyrimidinic endonuclease (chAPE1) expressed in sf9 cells reveals that its endonuclease activity is regulated by phosphorylation.中国仓鼠脱嘌呤/脱嘧啶核酸内切酶（chAPE1）在 sf9 细胞中的表达表明其核酸内切酶活性受磷酸化调节。
FEBS J. 2010 Nov;277(22):4732-40. doi: 10.1111/j.1742-4658.2010.07879.x. Epub 2010 Oct 19.
10
2'-deoxyribonolactone lesion produces G->A transitions in Escherichia coli.2'-脱氧核糖内酯损伤在大肠杆菌中产生G->A转换。
Nucleic Acids Res. 2004 May 24;32(9):2937-46. doi: 10.1093/nar/gkh622. Print 2004.
本文引用的文献
1
FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES.核酸及其组成核苷酸烷基化的进一步研究
Biochem J. 1963 Oct;89(1):127-38. doi: 10.1042/bj0890127.
2
Effect of uncouplers on radiosensitivity and mutagenicity in x-irradiated mammalian cells.解偶联剂对受X射线照射的哺乳动物细胞的辐射敏感性和诱变性的影响。
Proc Natl Acad Sci U S A. 1980 May;77(5):2702-5. doi: 10.1073/pnas.77.5.2702.
3
On the fidelity of DNA replication. The accuracy of Escherichia coli DNA polymerase I in copying natural DNA in vitro.论DNA复制的保真度。大肠杆菌DNA聚合酶I在体外复制天然DNA时的准确性。
J Biol Chem. 1980 Oct 25;255(20):9961-6.
4
The replication of DNA in murine lymphoma cells (L5178Y). I. Rate of replication.小鼠淋巴瘤细胞（L5178Y）中DNA的复制。I. 复制速率。
Biochim Biophys Acta. 1970 Mar 19;204(1):128-43. doi: 10.1016/0005-2787(70)90496-x.
5
Solvolyses of cytosine and cytidine.胞嘧啶和胞苷的溶剂解作用。
J Pharm Sci. 1972 Jul;61(7):1052-61. doi: 10.1002/jps.2600610703.
6
The properties of O 6 -methylguanine in templates for RNA polymerase.RNA聚合酶模板中O 6 -甲基鸟嘌呤的特性。
Biochim Biophys Acta. 1973 May 10;308(2):310-6. doi: 10.1016/0005-2787(73)90160-3.
7
Rate of depurination of native deoxyribonucleic acid.天然脱氧核糖核酸的脱嘌呤速率
Biochemistry. 1972 Sep 12;11(19):3610-8. doi: 10.1021/bi00769a018.
8
Heat-induced deamination of cytosine residues in deoxyribonucleic acid.脱氧核糖核酸中胞嘧啶残基的热诱导脱氨基作用。
Biochemistry. 1974 Jul 30;13(16):3405-10. doi: 10.1021/bi00713a035.
9
Commentary. Monofunctional alkylating agents and apurinic sites in DNA.
Biochem Pharmacol. 1974 Jan 1;23(1):3-8. doi: 10.1016/0006-2952(74)90307-4.
10
Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid.双链脱氧核糖核酸中脱嘌呤位点的链断裂速率。
Biochemistry. 1972 Sep 12;11(19):3618-23. doi: 10.1021/bi00769a019.
Zotero 插件