Chen Shi-Ying, Liu Wenkuan, Xu Yun, Qiu Shuyan, Chen Yong, Tian Xingui, Zhou Rong
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Front Microbiol. 2020 Dec 2;11:606195. doi: 10.3389/fmicb.2020.606195. eCollection 2020.
After the first outbreak in China in 2006, human adenovirus type 55 (HAdV-B55) has become a common pathogen causing life threatening pneumonia in northern China. However, HAdV-B55 infection has been rarely reported in southern China. Here, we collected throat swabs from 3,192 hospitalized children with acute respiratory disease (ARD) from May 2017 to April 2019 in Guangzhou, southern China, tested them for HAdV-B55 infection. Only one of 1,399 patients from May 2017 to April 2018 was HAdV-B55 positive; HAdV-B55 infections significantly increased with 10 of 1,792 patients testing positive since May 2018. HAdV-B55-267, isolated from a case of death, was sequenced for whole genomic analysis. Three other strains, HAdV-B55-Y16, -TY12, and -TY26, isolated earlier in patients from Shanxi, northern China, were also sequenced and analyzed. The four HAdV-B55 strains formed similar plaques, grew to similar titers, and resulted in similar typical cell pathogenic effects. HAdV-B55-267 formed a subclade with the prototype strain QS-DLL; strains HAdV-B55-Y16, -TY12, and -TY26 were closely related to strain QZ01. HAdV-B55 could be divided into two subtypes (HAdV-B55-a and -b) according to the presence or absence of the insertion of "CCATATCCGTGTT"; all strains isolated from China except for strain BJ01 belong to subtype b. HAdV-B55-267 had only one non-synonymous substitution comparing with strain QS-DLL, and all HAdV-B55 strains had highly conserved capsid proteins and few non-synonymous substitutions. This study suggests that HAdV-B55 is an important pathogen associated with ARD in Guangzhou since 2018, exhibiting the relative genome stability across time and geographic space in China.
2006年在中国首次爆发后，55型人腺病毒（HAdV-B55）已成为中国北方导致危及生命的肺炎的常见病原体。然而，中国南方很少有HAdV-B55感染的报道。在此，我们收集了2017年5月至2019年4月在中国南方广州3192例因急性呼吸道疾病（ARD）住院儿童的咽拭子，检测他们是否感染HAdV-B55。2017年5月至2018年4月的1399例患者中只有1例HAdV-B55呈阳性；自2018年5月以来，1792例患者中有10例检测呈阳性，HAdV-B55感染显著增加。从一例死亡病例中分离出的HAdV-B55-267进行了全基因组测序分析。另外三株分别从中国北方山西省患者中较早分离出的HAdV-B55-Y16、-TY12和-TY26也进行了测序和分析。这四株HAdV-B55毒株形成相似的噬菌斑，生长到相似的滴度，并产生相似的典型细胞致病效应。HAdV-B55-267与原型毒株QS-DLL形成一个亚分支；毒株HAdV-B55-Y16、-TY12和-TY26与毒株QZ01密切相关。根据是否存在“CCATATCCGTGTT”插入，HAdV-B55可分为两个亚型（HAdV-B55-a和-b）；除BJ01毒株外，所有从中国分离出的毒株都属于b亚型。与毒株QS-DLL相比，HAdV-B55-267只有一个非同义替换，所有HAdV-B55毒株的衣壳蛋白高度保守，非同义替换很少。这项研究表明，自2018年以来，HAdV-B55是广州与ARD相关的重要病原体，在中国的时间和地理空间上表现出相对的基因组稳定性。
