重新定义数字病理学的高倍视野

(Re) Defining the High-Power Field for Digital Pathology.

作者信息

Kim David, Pantanowitz Liron, Schüffler Peter, Yarlagadda Dig Vijay Kumar, Ardon Orly, Reuter Victor E, Hameed Meera, Klimstra David S, Hanna Matthew G

机构信息

Department of Pathology and Laboratory Medicine, New York-Presbyterian Hospital, Weill Cornell Medical College, New York, NY, USA.

Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.

出版信息

J Pathol Inform. 2020 Oct 9;11:33. doi: 10.4103/jpi.jpi_48_20. eCollection 2020.

DOI:10.4103/jpi.jpi_48_20

PMID:33343994

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7737490/

Abstract

BACKGROUND

The microscope high-power field (HPF) is the cornerstone for histopathology diagnostic evaluation such as the quantification of mitotic figures, lymphocytes, and tumor grading. With traditional light microscopy, HPFs are typically evaluated by quantifying histologic events in 10 fields of view at × 400 magnification. In the era of digital pathology, new variables are introduced that may affect HPF evaluation. The aim of this study was to determine the parameters that influence HPF in whole slide images (WSIs).

MATERIALS AND METHODS

Glass slides scanned on various devices (Leica's Aperio GT450, AT2, and ScanScope XT; Philips UltraFast Scanner; Hamamatsu's Nanozoomer 2.0HT; and 3DHistech's P1000) were compared to acquired digital slides reviewed on each vendor's respective WSI viewer software (e.g., Aperio ImageScope, ImageScope DX, Philips IMS, 3DHistech CaseViewer, and Hamamatsu NDP.view) and an in-house developed vendor-agnostic viewer. WSIs were reviewed at "×40" equivalent HPF on different sized monitors with varying display resolutions (1900 × 1080-4500 × 3000) and aspect ratios (e.g., Food and Drug Administration [FDA]-cleared 27" Philips PS27QHDCR, FDA-cleared 24" Dell MR2416, 24" Hewlett Packard Z24n G2, and 28" Microsoft Surface Studio). Digital and microscopic HPF areas were calculated and compared.

RESULTS

A significant variation of HPF area occurred between differing monitor size and display resolutions with minor differences between WSI viewers. No differences were identified by scanner or WSIs scanned at different resolutions (e.g., 0.5, 0.25, 0.24, and 0.12 μm/pixel).

CONCLUSION

Glass slide HPF at × 400 magnification with conventional light microscopy was not equivalent to "×40" digital HPF areas. Digital HPF quantification may vary due to differences in the tissue area displayed by monitor sizes, display resolutions, and WSI viewers but not by scanner or scanning resolution. These findings will need to be further clinically validated with potentially new digital metrics for evaluation.

摘要

背景

显微镜高倍视野（HPF）是组织病理学诊断评估的基石，如有丝分裂象、淋巴细胞的定量分析以及肿瘤分级。在传统光学显微镜下，通常通过在×400放大倍数下对10个视野中的组织学事件进行定量分析来评估HPF。在数字病理学时代，引入了可能影响HPF评估的新变量。本研究的目的是确定影响全切片图像（WSI）中HPF的参数。

材料与方法

将在各种设备（徕卡的Aperio GT450、AT2和ScanScope XT；飞利浦超快扫描仪；滨松的Nanozoomer 2.0HT；以及3DHistech的P1000）上扫描的玻璃切片与在每个供应商各自的WSI查看软件（如Aperio ImageScope、ImageScope DX、飞利浦IMS、3DHistech CaseViewer和滨松NDP.view）以及内部开发的与供应商无关的查看器上查看的采集数字切片进行比较。在不同尺寸的显示器上，以“×40”等效HPF对WSI进行查看，这些显示器具有不同的显示分辨率（1900×1080 - 4500×3000）和宽高比（如美国食品药品监督管理局[FDA]批准的27英寸飞利浦PS27QHDCR、FDA批准的24英寸戴尔MR2416、24英寸惠普Z24n G2和28英寸微软Surface Studio）。计算并比较数字和显微镜下的HPF面积。