Bacterial Disease Laboratory (LABAC), School of Veterinary Medicine and Zootechny, Federal University of Bahia, Adhemar de Barros - AV, 500, Ondina, Salvador, BA, 40170-11, Brazil.
Department of Preventive Veterinary Medicine and Animal Production, Universidade Federal da Bahia, Av. Adhemar de Barros, 500, Ondina, Salvador, BA, 40170-110, Brazil.
Ecohealth. 2022 Mar;19(1):15-21. doi: 10.1007/s10393-022-01577-9. Epub 2022 Feb 19.
Leptospirosis is an emerging zoonotic disease caused by bacteria of the genus Leptospira. Wild animals may present acute disease or become chronic hosts. The present study aimed to identify Leptospira spp. infection and determine circulating serogroups in free-ranging sambar deer (Rusa unicolor) in a fragment of peri-urban tropical forest in northeastern Brazil. Blood samples were collected and subjected to microscopic agglutination testing (MAT) and PCR analysis (genes lipL32 and secY). Anti-Leptospira antibodies were detected in 60% of the animals tested, with serogroups Cynopteri (33.4%), Hebdomadis (22.2%) and Grippotyphosa (22.2%) identified as most prevalent. DNA corresponding to the pathogenic species Leptospira interrogans was detected in 2/15 (13.3%) of the samples tested. Sambar deer experience a high level of exposure to Leptospira spp. in our epidemiological setting. It is important to emphasize the implementation of effective measures (i.e., maintaining habitats and reducing human contact) for the conservation of endangered species.
钩端螺旋体病是一种由钩端螺旋体属细菌引起的新兴人畜共患病。野生动物可能患有急性疾病或成为慢性宿主。本研究旨在鉴定自由放养的印度黑羚（Rusa unicolor）在巴西东北部城市周边热带森林片段中的钩端螺旋体属感染情况，并确定其循环血清群。采集血液样本并进行显微镜凝集试验（MAT）和 PCR 分析（lipL32 和 secY 基因）。在检测的动物中，60%检测到抗钩端螺旋体抗体，其中最常见的血清群为 Cynopteri（33.4%）、Hebdomadis（22.2%）和 Grippotyphosa（22.2%）。在检测的 15 个样本中，有 2 个（13.3%）样本检测到致病性物种问号钩端螺旋体的 DNA。在我们的流行病学环境中，印度黑羚接触钩端螺旋体属的水平很高。重要的是要强调实施有效的措施（即，维持栖息地和减少人类接触）来保护濒危物种。
