Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus 00970, State of Palestine.
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus 00970, State of Palestine.
Biomed Res Int. 2020 Dec 1;2020:8821319. doi: 10.1155/2020/8821319. eCollection 2020.
Medicinal plants are widely used in many cultures, traditions, and civilizations worldwide. Plants with high contents of the valuable biological compounds can efficiently cure many diseases. This study is aimed at assessing, for the first time, the anti--amylase, antilipase, antimicrobial, and cytotoxic activities of (Willd.) DC. of five extracts from Palestine.
The antimicrobial activity was estimated using well diffusion method for plant of five extracts against eight ATCC (American Type Culture Collection) and clinical isolates. The cytotoxic effects for these extracts were evaluated against HeLa (cervical) carcinoma cells using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Moreover, the lipase and -amylase inhibitory properties were determined using standard biomedical assays.
The acetone extract of plant showed a more potent -amylase inhibitory compared with acarbose with IC values of 25.7 ± 0.08 and 28.18 ± 1.22 g/ml, respectively. Additionally, the acetone and methanol extracts revealed moderate antilipase activity compared to orlistat with IC values of 30.19 ± 0.11, 33.11 ± 0.13, and 12.3 ± 0.35 g/ml, respectively. The methylene chloride extract was found to inhibit the growth of all the tested bacterial and fungal strains and also found to have potential cytotoxic effect against HeLa cancer cell line.
This research work reports for the first time the biological activity of from Palestine, and the results were promising indicating that extracts contain valuable bioactive molecules that have a potential anti--amylase, antilipase, antibacterial, and antifungal cytotoxic potentials. Therefore, could have a medical significance in the future.
药用植物在世界范围内的许多文化、传统和文明中被广泛使用。含有高含量有价值生物化合物的植物可以有效地治疗许多疾病。本研究旨在首次评估来自巴勒斯坦的 （Willd.） DC 的五种提取物的抗淀粉酶、脂肪酶、抗菌和细胞毒性活性。
使用琼脂扩散法评估五种植物提取物对八种 ATCC（美国典型培养物保藏中心）和临床分离株的抗菌活性。使用 MTS（3-（4,5-二甲基噻唑-2-基）-5-（3-羧甲氧基苯基）-2-（4-磺苯基）-2H-四唑）测定法评估这些提取物对 HeLa（宫颈）癌细胞的细胞毒性作用。此外，使用标准生物医学测定法测定脂肪酶和淀粉酶抑制特性。
与阿卡波糖相比，植物的丙酮提取物显示出更强的淀粉酶抑制作用，IC 值分别为 25.7±0.08 和 28.18±1.22 g/ml。此外，与奥利司他相比，丙酮和甲醇提取物显示出适度的脂肪酶抑制活性，IC 值分别为 30.19±0.11、33.11±0.13 和 12.3±0.35 g/ml。二氯甲烷提取物被发现抑制所有测试的细菌和真菌菌株的生长，并且对 HeLa 癌细胞系也具有潜在的细胞毒性作用。
本研究工作首次报道了来自巴勒斯坦的 的生物活性，结果令人鼓舞，表明 提取物含有有价值的生物活性分子，具有潜在的抗淀粉酶、脂肪酶、抗菌和抗真菌细胞毒性潜力。因此， 在未来可能具有医学意义。
