Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus P.O. Box 7, Palestine.
Molecules. 2022 Jul 18;27(14):4574. doi: 10.3390/molecules27144574.
is one of the traditional medicinal plants that grows in the arid area of the Al-Naqab desert in Palestine and is used by Bedouins to heal various communicable and non-communicable illnesses. The purpose of this investigation was to estimate the total phenolic, flavonoid, and tannin contents of aqueous, methanol, acetone, and hexane extracts and evaluate their cytotoxic, anti-oxidative, and antimicrobial properties. Qualitative phytochemical tests were used to identify the major phytochemical classes in extracts, while total phenol, flavonoid, and tannin contents were determined using Folin-Ciocalteu, aluminum chloride, and vanillin assays, respectively. Moreover, a microdilution test was employed to estimate the antimicrobial activity of extracts against several microbial species. At the same time, the cytotoxic and free radical scavenging effects were evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays, respectively. Quantitative examinations showed that the highest amounts of phenols, flavonoids, and tannins were noticed in the aqueous extract. Moreover, aqueous extract showed potent activity against methicillin-resistant even more than Amoxicillin and Ofloxacin antibiotics, with Minimum Inhibitory Concentrations (MICs) of 0.78 ± 0.01, 0, and 1.56 ± 0.03 µg/mL, respectively. Additionally, the aqueous extract exhibited the highest activity against and pathogens, with MIC values of 0.78 ± 0.01 µg/mL. Actually, the aqueous extract showed more potent antimold activity than Ketoconazole against with MICs of 0.78 ± 0.01 and 1.56 ± 0.02 µg/mL, respectively. Furthermore, all extracts showed potential cytotoxic effects against breast cancer (MCF-7), hepatocellular carcinoma (Hep 3B and Hep G2), and cervical adenocarcinoma (HeLa) tumor cell lines. In addition, the highest free radical scavenging activity was demonstrated by aqueous extract compared with Trolox with IC doses of 6.16 ± 0.75 and 2.23 ± 0.57 µg/mL, respectively. Studying aqueous extract could lead to the development of new treatments for diseases such as antibiotic-resistant microbes and cancer, as well as for oxidative stress-related disorders such as oxidative stress. aqueous extract may help in the design of novel natural preservatives and therapeutic agents.
是生长在巴勒斯坦内盖夫沙漠干旱地区的传统药用植物之一，被贝都因人用于治疗各种传染病和非传染性疾病。本研究的目的是评估水提物、甲醇提取物、丙酮提取物和正己烷提取物的总酚、类黄酮和单宁含量，并评价它们的细胞毒性、抗氧化和抗菌特性。采用定性植物化学试验鉴定提取物中的主要植物化学类，同时采用福林-肖卡(Folin-Ciocalteu)、三氯化铝和香草醛法分别测定总酚、类黄酮和单宁含量。此外，采用微量稀释法测定提取物对几种微生物的抗菌活性。同时，采用 3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑鎓(MTS)和 2,2-二苯基-1-苦基肼水合物(DPPH)法分别评价提取物的细胞毒性和自由基清除活性。定量研究表明，水提物中酚类、类黄酮和单宁的含量最高。此外，水提物对耐甲氧西林金黄色葡萄球菌的活性甚至强于阿莫西林和氧氟沙星抗生素，最低抑菌浓度（MIC）分别为 0.78 ± 0.01、0 和 1.56 ± 0.03 µg/mL。此外，水提物对 和 病原体的活性最高，MIC 值分别为 0.78 ± 0.01 µg/mL。实际上，水提物对 曲霉菌的抗真菌活性比酮康唑更强，MIC 值分别为 0.78 ± 0.01 和 1.56 ± 0.02 µg/mL。此外，所有提取物对乳腺癌（MCF-7）、肝癌（Hep 3B 和 Hep G2）和宫颈腺癌（HeLa）肿瘤细胞系均表现出潜在的细胞毒性作用。此外，水提物的自由基清除活性最高，与 Trolox 相比，IC 剂量分别为 6.16 ± 0.75 和 2.23 ± 0.57 µg/mL。研究 水提物可能有助于开发治疗抗生素耐药微生物和癌症等疾病以及与氧化应激相关的疾病如氧化应激的新疗法。水提物可能有助于设计新型天然防腐剂和治疗剂。
