光滑球拟酵母的准性遗传学
Parasexual genetics of Torulopsis glabrata.
作者信息
Whelan W L, Kwon-Chung K J
机构信息
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
出版信息
J Bacteriol. 1987 Nov;169(11):4991-4. doi: 10.1128/jb.169.11.4991-4994.1987.
Abstract
Prototrophic hybrids were generated in the asexual yeast Torulopsis glabrata by the fusion of spheroplasts derived from parent strains which bore complementing auxotrophic markers. The DNA content (per cell) of two hybrids was essentially that predicted by summing the corresponding parental values. UV irradiation of these two hybrids resulted in the formation of sectored colonies with genetic properties consistent with their origin by either mitotic recombination or chromosomal nondisjunction.
摘要
通过融合来自带有互补营养缺陷型标记的亲本菌株的原生质体，在无性酵母光滑球拟酵母中产生了原养型杂种。两个杂种的DNA含量（每细胞）基本上是通过将相应亲本值相加所预测的数值。对这两个杂种进行紫外线照射后，形成了具有与其起源相符的遗传特性的扇形菌落，其起源方式为有丝分裂重组或染色体不分离。
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