Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, West Virginia 26506-6106, United States.
Department of Chemical Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003-9303, United States.
Anal Chem. 2021 Jan 26;93(3):1326-1332. doi: 10.1021/acs.analchem.0c02555. Epub 2020 Dec 21.
Conventional paper lateral flow assays have low sensitivity and suffer from severe interference from complex human fluid sample matrices, which prevents their practical application in the testing of whole blood samples in the point-of-care settings. To solve this problem, gold nanostar@Raman reporter@silica-sandwiched nanoparticles have been developed as the surface-enhanced Raman scattering (SERS) probes for sensing transduction; and a functionalized filter membrane assembly has been designed and constructed in the paper-based lateral flow strip (PLFS) as a built-in plasma separation unit. In this "on-strip" plasma separation unit, three layers of filter membranes are stacked and surface-modified to maximize the separation efficiency and the plasma yield. As a result, the integrated PLFS has been successfully used for the detection of carcinoembryonic antigen (CEA) in 30 μL of whole blood with the assistance of a portable Raman reader, achieving a limit of detection of 1.0 ng mL. In short, this report presents an inexpensive, disposable, portable, and field-deployable paper-based device as a general point-of-care testing tool for protein biomarker detection in a drop of whole blood.
传统的纸质横向流动分析物检测灵敏度低，并且容易受到复杂的人体流体样本基质的严重干扰，这限制了它们在即时护理环境中对全血样本的实际应用。为了解决这个问题，已经开发了金纳米星@拉曼报告分子@二氧化硅夹心纳米颗粒作为表面增强拉曼散射（SERS）探针进行传感转换；并且在基于纸的横向流动条（PLFS）中设计并构建了功能化的滤膜组件作为内置的等离子体分离单元。在这个“片上”等离子体分离单元中，堆叠并表面修饰了三层滤膜，以最大程度地提高分离效率和等离子体产量。因此，集成的 PLFS 已成功用于在便携式 Raman 读取器的辅助下检测 30 μL 全血中的癌胚抗原（CEA），检测限达到 1.0ng mL。简而言之，本报告提出了一种廉价、一次性、便携式且可现场部署的基于纸张的设备，可作为一种通用的即时护理检测工具，用于检测一滴全血中的蛋白质生物标志物。
