一种新合成的基于双（萨拉莫）的视觉荧光化学传感器，用于在活细胞和斑马鱼中对外源性硼进行检测。
A newly synthesized visual bis(salamo)-based fluorescent chemosensor for exogenous detection of BO in living cells and zebrafish.
作者信息
Pu Lu-Mei, Wang Lan, Wei Zhi-Li, Chen Zhuang-Zhuang, Long Hai-Tao, Ru Jia-Xi, Dong Xiu-Yan
机构信息
College of Science, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 15;249:119263. doi: 10.1016/j.saa.2020.119263. Epub 2020 Nov 28.
A newly synthesized fluorescent chemosensor HL was explored for detecting BO, characterized by H NMR spectrum, mass spectrum and fluorescence spectra. During the detection process of BO, the fluorescence is significantly enhanced and naked eye recognition can be performed under 365 nm UV light without any interference by other typical anions. The limit of detection is as low as 6.97 × 10 M. In addition, in order to broaden the application of salamo-based fluorescence sensors in the field of biology, except for the fluorescence imaging of HeLa cells, the first attempt of exogenous detection in zebrafish was conducted successfully.
一种新合成的荧光化学传感器HL被用于检测硼酸盐，通过核磁共振氢谱、质谱和荧光光谱对其进行了表征。在硼酸盐的检测过程中，荧光显著增强，并且在365 nm紫外光下可进行肉眼识别，不受其他典型阴离子的任何干扰。检测限低至6.97×10⁻⁶ M。此外，为了拓宽基于萨洛莫的荧光传感器在生物学领域的应用，除了对HeLa细胞进行荧光成像外，还成功地首次尝试了在斑马鱼体内进行外源检测。
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