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除微囊藻毒素之外：蓝藻提取物诱导水稻根细胞骨架改变。

Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells.

机构信息

Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece.

出版信息

Int J Mol Sci. 2020 Dec 17;21(24):9649. doi: 10.3390/ijms21249649.

DOI:10.3390/ijms21249649
PMID:33348912
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7766381/
Abstract

Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far been focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and HO levels in (rice) root cells. Seedling roots were treated for various time periods (1, 12, and 24 h) with aqueous cyanobacterial extracts and underwent either immunostaining for -tubulin or staining of F-actin with fluorescent phalloidin. 2,7-dichlorofluorescein diacetate (DCF-DA) staining was performed for HO imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC-) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

摘要

微囊藻毒素（MCs）是蓝藻毒素，也是蛋白磷酸酶 1（PP1）和 2A（PP2A）的有效抑制剂，这两种酶参与植物细胞骨架（微管和 F-肌动蛋白）的组织。因此，目前关于蓝藻产物对植物细胞毒性的研究主要集中在 MCs 上。在这项研究中，我们研究了来自多个栖息地的 16 株（4 株产 MC 和 12 株非产 MC）蓝藻菌株的提取物对各种酶（PP1、胰蛋白酶、弹性蛋白酶）、植物细胞骨架和水稻根细胞中 HO 水平的影响。用含蓝藻的水提取物处理幼苗根 1、12 和 24 小时，并用 -微管蛋白免疫染色或荧光鬼笔环肽染色 F-肌动蛋白，进行 HO 成像的 2,7-二氯荧光素二乙酸酯（DCF-DA）染色。酶分析证实了 MC 丰富（MC+）和 MC 缺乏（MC-）提取物的生物活性，这些提取物诱导了植物细胞骨架两个成分的主要时间依赖性改变。这些发现表明，除了 MC 或其他已知的蓝藻毒素（如节旋螺毒素）之外，广泛存在的生物活性蓝藻化合物可以通过破坏细胞骨架来影响植物。

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