Kallioniemi O P, Punnonen R, Mattila J, Lehtinen M, Koivula T
Department of Clinical Chemistry, Tampere University Central Hospital, Finland.
Cancer. 1988 Jan 15;61(2):334-9. doi: 10.1002/1097-0142(19880115)61:2<334::aid-cncr2820610224>3.0.co;2-m.
Paraffin-embedded tumor samples from 157 ovarian cancer patients were analyzed by DNA flow cytometry. Tumor ploidy had prognostic significance in both early and advanced ovarian cancer. After adjusting for stage, residual tumor mass, histopathologic type, treatment, and age of patient in a Cox regression analysis, the relative risk of death was two-fold higher in single DNA-aneuploid and sixfold higher in DNA-multiploid tumors as compared to DNA-diploid tumors (P less than 0.0001). A tetraploid DNA index was associated with a relatively low risk ratio, whereas hypertetraploid tumors were highly malignant. S-phase fraction (SPF) had prognostic value especially in DNA-diploid tumors. The simultaneous evaluation of DNA index, the number of aneuploid cell clones, and SPF gave more prognostic information than the determination of tumor ploidy alone. On the basis of these parameters we have developed a classification of tumor DNA histograms for better prognostic assessment of ovarian cancer.
对157例卵巢癌患者的石蜡包埋肿瘤样本进行了DNA流式细胞术分析。肿瘤倍性在早期和晚期卵巢癌中均具有预后意义。在Cox回归分析中，校正分期、残余肿瘤大小、组织病理学类型、治疗及患者年龄后，与DNA二倍体肿瘤相比，单倍体DNA非整倍体肿瘤的相对死亡风险高2倍，DNA多倍体肿瘤高6倍（P<0.0001）。四倍体DNA指数与相对较低的风险比相关，而超四倍体肿瘤具有高度恶性。S期分数（SPF）具有预后价值，尤其在DNA二倍体肿瘤中。同时评估DNA指数、非整倍体细胞克隆数和SPF比单独测定肿瘤倍性能提供更多的预后信息。基于这些参数，我们制定了肿瘤DNA直方图分类法，以更好地评估卵巢癌的预后。
