ICAR - National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, Uttar Pradesh- 275103, India.
Ginning Training Centre, ICAR - Central Institute for Research on Cotton Technology (CIRCOT), Nagpur, Maharashtra - 440023, India.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004611. Epub 2020 Dec 22.
A moderately halophilic, Gram-stain-negative, aerobic bacterium, strain D1-1, belonging to the genus , was isolated from soil sampled at Pentha beach, Odisha, India. Phylogenetic trees reconstructed based on 16S rRNA genes and multilocus sequence analysis of and genes revealed that strain D1-1 belonged to the genus and was most closely related to YKJ-16 (98.1 %) followed by Al12 (97.5 %), CPS11 (97.5 %), 5CR (97.4 %) and DSM 735 (97.2 %) on the basis of 16S rRNA gene sequence similarity. Sequence identities with other species within the genus were lower than 97.0 %. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of 22.4-30 % and 79.5-85.4 % with close relatives of DSM 735, . YKJ-16, Al12 and 5CR were lower than the threshold recommended for species delineation (70 % and 95-96 % for dDDH and ANI, respectively). Further, strain D1-1 formed yellow-coloured colonies; cells were rod-shaped, motile with optimum growth at 30 °C (range, 4-45 °C) and 2-8 % NaCl (w/v; grew up to 24 % NaCl). The major fatty acids were summed feature 8 (C 7/C 6), summed feature 3 (C 7/C 6) and C and the main respiratory quinone was ubiquinone Q-9 in line with description of the genus. Based on its chemotaxonomic and phylogenetic characteristics and genome uniqueness, strain D1-1 represents a novel species in the genus , for which we propose the name sp. nov., within the family . The type strain is D1-1 (=JCM 33602=KACC 21317=NAIMCC-B-2254).
一株中度嗜盐、革兰氏阴性、需氧细菌，菌株 D1-1，分离自印度奥里萨邦彭塔海滩的土壤。基于 16S rRNA 基因和 基因的多位序列分析构建的系统发育树表明，菌株 D1-1 属于 属，与 YKJ-16（98.1%）最为密切相关，其次是 Al12（97.5%）、CPS11（97.5%）、5CR（97.4%）和 DSM 735（97.2%），基于 16S rRNA 基因序列相似性。与属内其他种的序列同一性低于 97.0%。与近缘种 DSM 735、YKJ-16、Al12 和 5CR 的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值分别为 22.4-30%和 79.5-85.4%，低于种间区分的建议阈值（分别为 70%和 95-96%的 dDDH 和 ANI）。此外，菌株 D1-1 形成黄色菌落；细胞呈杆状，运动性强，最适生长温度为 30°C（范围为 4-45°C）和 2-8%NaCl（w/v；可生长至 24%NaCl）。主要脂肪酸为 C 7/C 6 总和特征 8、C 7/C 6 总和特征 3 和 C，主要呼吸醌为 ubiquinone Q-9，符合属的描述。基于其化学分类学和系统发育特征以及基因组的独特性，菌株 D1-1 代表 属的一个新种，我们建议将其命名为 sp. nov.，属于 科。模式菌株为 D1-1（=JCM 33602=KACC 21317=NAIMCC-B-2254）。
