Frosini Sian Marie, Bond Ross, McCarthy Alex J, Feudi Claudia, Schwarz Stefan, Lindsay Jodi A, Loeffler Anette
Department of Clinical Science and Services, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK.
MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UK.
Microorganisms. 2020 Dec 18;8(12):2031. doi: 10.3390/microorganisms8122031.
Transmission of methicillin-resistant (MRSA) and methicillin-resistant (MRSP) between people and pets, and their co-carriage, are well-described. Potential exchange of antimicrobial resistance (AMR) genes amongst these staphylococci was investigated in vitro through endogenous bacteriophage-mediated transduction. Bacteriophages were UV-induced from seven donor isolates of canine (MRSP) and human (MRSA) origin, containing (M), (K), or , and lysates filtered. Twenty-seven tetracycline- and fusidic acid- (FA-) susceptible recipients were used in 122 donor-recipient combinations (22 tetracycline, 100 FA) across 415 assays (115 tetracycline, 300 FA). Bacteriophage lysates were incubated with recipients and presumed transductants quantified on antimicrobial-supplemented agar plates. Tetracycline resistance transduction from MRSP and MRSA to methicillin-susceptible (MSSP) was confirmed by PCR in 15/115 assays. No FA-resistance transfer occurred, confirmed by negative PCR, but colonies resulting from FA assays had high MICs (≥32 mg/L) and showed mutations in , two at a novel position (F88L), nine at H457[Y/N/L]. Horizontal gene transfer of tetracycline-resistance confirms that resistance genes can be shared between coagulase-positive staphylococci from different hosts. Cross-species AMR transmission highlights the importance of good antimicrobial stewardship across humans and veterinary species to support One Health.
耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林凝固酶阴性葡萄球菌（MRSP）在人与宠物之间的传播及其共同携带情况已有详尽描述。通过内源性噬菌体介导的转导在体外研究了这些葡萄球菌之间抗菌药物耐药性（AMR）基因的潜在交换。从7株犬源（MRSP）和人源（MRSA）的供体分离株中通过紫外线诱导出噬菌体，这些分离株含有（M）、（K）或 ，并对裂解物进行过滤。在415次试验（115次四环素试验、300次夫西地酸试验）中，使用了27株对四环素和夫西地酸（FA）敏感的受体菌，进行了122种供体 - 受体组合试验（22次四环素试验、100次FA试验）。将噬菌体裂解物与受体菌一起孵育，并在添加抗菌药物的琼脂平板上对假定的转导子进行定量。在115次试验中的15次试验中，通过PCR证实了四环素耐药性从MRSP和MRSA转导至甲氧西林敏感的凝固酶阴性葡萄球菌（MSSP）。PCR结果为阴性，证实未发生FA耐药性转移，但FA试验产生的菌落具有高最低抑菌浓度（≥32 mg/L），并且在 中显示出突变，其中两个在一个新位置（F88L），九个在H457[Y/N/L]。四环素耐药性的水平基因转移证实了耐药基因可以在来自不同宿主的凝固酶阳性葡萄球菌之间共享。跨物种AMR传播凸显了在人类和兽医学物种中做好抗菌药物管理以支持“同一健康”理念的重要性。
