Nie Chan, Yu Huiting, Wang Xue, Li Xiahong, Wei Zairong, Shi Xiuquan
Department of Epidemiology and Health Statistics, School of Public Health, Zunyi Medical University, Zunyi, Guizhou, China.
Department of Epidemiology and Health Statistics, School of Public Health, Guizhou Medical University, Guiyang, Guizhou, China.
PeerJ. 2020 Dec 8;8:e10499. doi: 10.7717/peerj.10499. eCollection 2020.
A burn is an inflammatory injury to the skin or other tissue due to contact with thermal, radioactive, electric, or chemical agents. Burn injury is an important cause of disability and death worldwide. Obesity is a significant public health problem, often causing underlying systemic inflammation. Studying the combined impact of burn injuries on obese patients has become critical to the successful treatment of these patients. The aim of this paper is to highlight the effect of inflammation associated with burn injuries on several body weight group in a rat study.
Different degrees of obesity and burns were established in rats and divided into a normal weight group, overweight group, obese group, second-degree burn group, third-degree burn group, over-weight second-degree burn group, over-weight third-degree burn group, obese second-degree burn group, and obese third-degree burn group (20 rats per group). Changes in inflammatory factors and growth factor were measured on the 1st, 3rd, 7th and 14th days after burns were inflicted.
The ELISA test showed that in the unburned control group, MCP-1, IL-1β and TNF-α protein expressions in the obese and over-weight groups were higher than the normal-weight group ( < 0.05). RT-PCR test showed that the expressions of MCP-1, IL-1β and TNF-α genes in the obese group were higher compared to the overweight and normal weight groups ( < 0.05). Three and 7 days after burns were inflicted, the level of VEGF in the normal weight group was higher than the obese group ( < 0.05), however increased VEGF was not observed on days 1 and 14.
Burn injury and obesity have a mutually synergistic effect on the body's inflammatory response.
烧伤是由于接触热、放射性、电或化学物质而导致的皮肤或其他组织的炎症性损伤。烧伤是全球范围内致残和死亡的重要原因。肥胖是一个重大的公共卫生问题，常引发潜在的全身炎症。研究烧伤对肥胖患者的综合影响已成为成功治疗这些患者的关键。本文旨在强调在一项大鼠研究中，烧伤相关炎症对几个体重组的影响。
在大鼠中建立不同程度的肥胖和烧伤模型，并分为正常体重组、超重组、肥胖组、二度烧伤组、三度烧伤组、超重二度烧伤组、超重三度烧伤组、肥胖二度烧伤组和肥胖三度烧伤组（每组20只大鼠）。在烧伤后的第1、3、7和14天测量炎症因子和生长因子的变化。
ELISA检测显示，在未烧伤的对照组中，肥胖组和超重组的MCP-1、IL-1β和TNF-α蛋白表达高于正常体重组（<0.05）。RT-PCR检测显示，肥胖组的MCP-1、IL-1β和TNF-α基因表达高于超重组和正常体重组（<0.05）。烧伤后3天和7天，正常体重组的VEGF水平高于肥胖组（<0.05），但在第1天和第14天未观察到VEGF升高。
烧伤和肥胖对机体的炎症反应具有相互协同作用。
