Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran.
J Biomed Mater Res B Appl Biomater. 2021 Sep;109(9):1259-1270. doi: 10.1002/jbm.b.34786. Epub 2020 Dec 22.
The aim of this study was to prepare a porous scaffold out of 58S bioactive glass as the bare and coated with Zein to improve mechanical properties and acting as a carrier for Kaempferol controlled delivery. Porosity and morphology, mechanical properties, drug release behavior, bioactivity, cell attachment, and biodegradation of the scaffolds were evaluated accordingly. Obtained results indicated that the scaffolds coated by (7wt/v %) Zein solution, showed the highest mechanical strength (3.06 ± 0.4 MPa) and desirable porous morphology. These scaffolds could support bioactivity, cell attachment, and provide sustained drug release in the safe range of Kaempferol concentration confirmed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis. Overall, this study showed that the Zein-coated scaffold possesses superior properties rather than bare scaffold, and the scaffolds coated with 7wt/v % Zein solution could be considered as appropriate scaffolds for bone regeneration.
本研究旨在制备一种多孔支架，由 58S 生物活性玻璃制成，裸露或涂有玉米醇溶蛋白，以提高机械性能，并作为载体制备山奈酚的控释剂。相应地评估了支架的多孔性和形态、机械性能、药物释放行为、生物活性、细胞附着和生物降解。研究结果表明，用（7wt/v%）玉米醇溶蛋白溶液涂覆的支架具有最高的机械强度（3.06±0.4 MPa）和理想的多孔形态。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐分析证实，这些支架可以支持生物活性、细胞附着，并提供山奈酚浓度在安全范围内的持续药物释放。总的来说，本研究表明，玉米醇溶蛋白涂层支架具有比裸露支架更优异的性能，并且涂覆 7wt/v%玉米醇溶蛋白溶液的支架可以被认为是适合骨再生的支架。
