Amano J, Nishimura R, Mochizuki M, Kobata A
Department of Biochemistry, University of Tokyo, Japan.
J Biol Chem. 1988 Jan 25;263(3):1157-65.
Human chorionic gonadotropins (hCGs) highly purified from the urine of patients with trophoblastic diseases and of healthy pregnant women contain approximately four mucin-type sugar chains in one molecule. The structures of these sugar chains were studied comparatively by using a new sensitive method to obtain mucin-type sugar chains quantitatively as radioactive oligosaccharides from a small amount of glycoproteins. The mucin-type sugar chains of all hCGs include sialylated and nonsialylated Gal beta 1----3GalNAc and Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAc. In the case of normal hCG and hydatidiform mole hCG, oligosaccharides containing the tetrasaccharide core occupy approximately 10% of the total mucin-type sugar chains. The ratio of the tetrasaccharide containing oligosaccharides is increased prominently to approximately 60% in choriocarcinoma hCG. The proportion in invasive mole hCG was also increased, but less than the proportion of choriocarcinoma hCG.
从滋养层疾病患者及健康孕妇尿液中高度纯化得到的人绒毛膜促性腺激素（hCG），一个分子中大约含有四条粘蛋白型糖链。通过一种新的灵敏方法对这些糖链的结构进行了比较研究，该方法可从少量糖蛋白中定量获得作为放射性寡糖的粘蛋白型糖链。所有hCG的粘蛋白型糖链均包含唾液酸化和非唾液酸化的Galβ1----3GalNAc以及Galβ1----4GlcNAcβ1----6（Galβ1----3）GalNAc。对于正常hCG和葡萄胎hCG而言，含有四糖核心的寡糖约占总粘蛋白型糖链的10%。在绒毛膜癌hCG中，含四糖寡糖的比例显著增加至约60%。侵袭性葡萄胎hCG中的比例也有所增加，但低于绒毛膜癌hCG中的比例。
