Kaczmarek Adam, Rocka Anna, Wesołowska Maria, Gołąb Elżbieta, Sałamatin Rusłan
Faculty of Medicine, Collegium Medicum, Cardinal Stefan Wyszyński University in Warsaw, ul. Kazimierza Wóycickiego 1/3, 01-938 Warsaw, Poland.
W. Stefański Institute of Parasitology, Polish Academy of Sciences, ul. Twarda 51/55, 00-818 Warsaw, Poland.
Ann Parasitol. 2020;66(4):573-579. doi: 10.17420/ap6604.300.
Blastocystis cf. hominis is an unicellular protozoan parasite commonly found in the gastrointestinal tract of humans and animals. Blastocystis is characterized by high morphological and genetic diversity. Studies based on the analysis of Blastocystis spp. small subunit ribosomal RNA genes (SSU rDNA) have identified 26 subtypes (ST) so far, including at least 10 isolated from humans (STs 1-9 and ST12). In 2017, stool samples from a dog and its two owners living in Gdynia, Poland were examined; all three were suffering from chronic diarrhoea. In addition, 30 faecal samples were also examined from 30 dogs kept in one of Warsaw's hotels for animals. Stool specimens were analyzed using anaerobic cultivation at 37°C with a modified Jones' medium and molecular methods (PCR). Phylogenetic analysis using Bayesian inference was performed. Vacuolar forms of Blastocystis were identified in the stool samples of the dog and its owners; Blastocystis were not detected in any sample from the dogs living in the animal hotel. Based on the phylogenetic analysis, the obtained isolates were classified as subtype ST3 (for Owner 1) and subtype ST7 (for Owner 2 and the dog). To the best of our knowledge, the present study is the first to evaluate the presence of Blastocystis in canines in Poland, including domestic dogs.
人芽囊原虫（未定种）是一种单细胞原生动物寄生虫，常见于人和动物的胃肠道。芽囊原虫具有高度的形态和遗传多样性。基于对芽囊原虫小亚基核糖体RNA基因（SSU rDNA）分析的研究迄今已鉴定出26个亚型（ST），其中至少10个亚型是从人类中分离出来的（ST1 - 9和ST12）。2017年，对来自波兰格但斯克的一只狗及其两名主人的粪便样本进行了检测；这三人都患有慢性腹泻。此外，还对在华沙一家动物酒店饲养的30只狗的30份粪便样本进行了检测。粪便标本采用改良琼斯培养基在37°C下进行厌氧培养并结合分子方法（PCR）进行分析。采用贝叶斯推断进行系统发育分析。在狗及其主人的粪便样本中鉴定出了空泡型芽囊原虫；在动物酒店饲养的狗的任何样本中均未检测到芽囊原虫。基于系统发育分析，获得的分离株被分类为ST3亚型（主人1）和ST7亚型（主人2和狗）。据我们所知，本研究是首次评估波兰犬类（包括家犬）中芽囊原虫的存在情况。
