Kaczmarek Adam, Sobociński Wojciech, Wesołowska Maria, Gołąb Elżbieta, Kołodziej-Sobocińska Marta, Sałamatin Rusłan
Faculty of Medicine. Collegium Medicum, Cardinal Stefan Wyszyński University in Warsaw, Kazimierza Wóycickiego 1/3, 01-938 Warsaw, Poland.
Institute of Biology, University of Białystok, Ciołkowskiego 1J, 15-245 Białystok, Poland.
Int J Parasitol Parasites Wildl. 2021 Sep 1;16:120-125. doi: 10.1016/j.ijppaw.2021.08.010. eCollection 2021 Dec.
cf. is the most common unicellular parasite found in human and animal intestines. Little is known about the life cycle, transmission and mechanisms of pathogenesis for this parasite. The aim of this study was to obtain new data on the genetic diversity of in selected species of mammals found in the Białowieża Primeval Forest (BPF), Poland, the best preserved lowland forest in temperate Europe. 113 faecal samples were collected in the period 2018-2020 from seven species of wild mammals occurring within the Polish part of BPF and its surroundings. was detected by molecular amplification and sequencing the small subunit rRNA gene. The overall prevalence of in animals was 8.9%. A larger number of stool samples (90) were collected from European bison and only for this species was it possible to calculate the prevalence of infection (5.6%). The isolates obtained from European bison were classified as ST1, ST3, ST5, ST7. was also detected in the grey wolf () (ST5), wild boar () (ST5) and red deer () (ST1). In conclusion, for the first time we have demonstrated the presence of in wild European bison. Preliminary studies have shown that is present in at least 4 species of wild mammals in the BPF area and that it exhibits great genetic diversity.
隐孢子虫是在人类和动物肠道中发现的最常见的单细胞寄生虫。关于这种寄生虫的生命周期、传播和发病机制知之甚少。本研究的目的是获取有关波兰比亚沃维耶扎原始森林（BPF）中选定哺乳动物物种隐孢子虫遗传多样性的新数据，BPF是温带欧洲保存最完好的低地森林。2018年至2020年期间，从BPF波兰部分及其周边地区出现的七种野生哺乳动物中收集了113份粪便样本。通过对小亚基rRNA基因进行分子扩增和测序来检测隐孢子虫。动物中隐孢子虫的总体患病率为8.9%。从欧洲野牛收集了更多数量的粪便样本（90份），并且仅针对该物种计算了感染率（5.6%）。从欧洲野牛获得的分离株被分类为ST1、ST3、ST5、ST7。在灰狼（ST5）、野猪（ST5）和马鹿（ST1）中也检测到了隐孢子虫。总之，我们首次证明了野生欧洲野牛中存在隐孢子虫。初步研究表明，BPF地区至少4种野生哺乳动物中存在隐孢子虫，并且它表现出很大的遗传多样性。
