动态巯基/二硫键平衡作为糖尿病酮症酸中毒的氧化应激标志物。
Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis.
机构信息
Department of Emergency Medicine, Ankara City Hospital, Ankara, Turkey
Department of Emergency Medicine, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
出版信息
Turk J Med Sci. 2021 Apr 30;51(2):743-748. doi: 10.3906/sag-1904-55.
BACKGROUND/AIM: The aim of present study was to investigate the dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis (DKA).
MATERIALS AND METHODS
A total of 77 participants consisting of 32 patients with DKA and 45 healthy volunteers were included in the study. Thiol/disulfide homeostasis (TDH) [total thiol-native thiol/disulfide changes] were measured in both groups (patient group and control group) using a brand new method developed by Erel and Neselioglu. Half of the difference between total thiol and native thiol concentrations gives the amount of disulfide bond.
RESULTS
Total thiol, native thiol, and disulfide levels in blood were found to be low. The levels of total thiol (P < 0.001) and native thiol (P < 0.001 ) were significantly lower in patients with DKA than in the control group. At the same time, the level of disulfide was nonsignificantly lower in the patient group than the control group (P = 0.388). The level of IMA was higher in the patient group than in the control group (P < 0.001).
CONCLUSION
The total thiol, native thiol, and disulfide levels in DKA decrease in favor of oxidative stress.
背景/目的：本研究旨在探讨糖尿病酮症酸中毒（DKA）患者氧化应激标志物——巯基/二硫键动态平衡的变化。
材料和方法
本研究共纳入 77 名参与者，包括 32 名 DKA 患者和 45 名健康志愿者。使用 Erel 和 Neselioglu 开发的全新方法，分别测量两组（患者组和对照组）的巯基/二硫键动态平衡（TDH）[总巯基-天然巯基/二硫键变化]。总巯基和天然巯基浓度之间的一半差异给出二硫键的数量。
结果
血液中的总巯基、天然巯基和二硫化物水平较低。与对照组相比，DKA 患者的总巯基（P<0.001）和天然巯基（P<0.001）水平显著降低。与此同时，患者组中二硫键的水平与对照组相比无显著差异（P=0.388）。与对照组相比，患者组的 IMA 水平更高（P<0.001）。
结论
DKA 患者的总巯基、天然巯基和二硫键水平下降，有利于氧化应激。
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