State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmacy Sciences, Guangxi Normal University, Guilin 541004, P. R. China.
Langmuir. 2021 Jan 12;37(1):569-577. doi: 10.1021/acs.langmuir.0c03290. Epub 2020 Dec 24.
The integration of surface-enhanced Raman spectrum (SERS) and fluorescence-photoacoustic multimodal imaging in near-infrared photothermal therapy is highly desirable for cancer theranostic. However, typically, gold nanotheranostics usually require an additional modification of fluorophores and complex design refinements. In this work, by integrating surface-modified cysteine-hydroxyl merocyanine (CyHMC) molecules onto AuNRs, a novel lysosome-targeted gold-based nanotheranostics AuNRs-CyHMC that combines the specificity of Raman spectrum, the speed of fluorescence imaging, and deep penetration of photoacoustic imaging was successfully fabricated. Interestingly, fluorescence and Raman signals in this AuNRs-CyHMC system do not interfere, but it has pH-sensitive Raman signals and self-fluorescence localization ability under different excitation wavelengths. Fluorescence co-localization experiments further confirmed the lysosome-targeting ability of AuNRs-CyHMC. Typically, the proposed nanotheranostics were capable of SERS monitoring pH changes in both phosphate-buffered saline and living cells. Meanwhile, and experiments revealed that AuNRs-CyHMC possessed excellent fluorescence-photoacoustic performance and could be used for multimodal imaging-guided photothermal therapy. Furthermore, our work implied that gold nanotheranostics can provide great potential for cancer diagnosis and treatment.
表面增强拉曼光谱（SERS）与荧光-光声多模态成像在近红外光热治疗中的集成对于癌症的诊断与治疗极具吸引力。然而，通常情况下，金纳米诊疗剂通常需要对荧光团进行额外的修饰和复杂的设计改进。在这项工作中，通过将表面修饰的半胱氨酸-羟基甲川菁（CyHMC）分子整合到 AuNRs 上，成功制备了一种新型的溶酶体靶向金基纳米诊疗剂 AuNRs-CyHMC，它结合了拉曼光谱的特异性、荧光成像的速度和光声成像的深穿透性。有趣的是，在这个 AuNRs-CyHMC 系统中，荧光和拉曼信号互不干扰，但它具有 pH 敏感的拉曼信号和在不同激发波长下的自荧光定位能力。荧光共定位实验进一步证实了 AuNRs-CyHMC 的溶酶体靶向能力。通常，所提出的纳米诊疗剂能够在磷酸盐缓冲液和活细胞中监测 SERS 下的 pH 变化。同时，细胞摄取和体内实验揭示了 AuNRs-CyHMC 具有优异的荧光-光声性能，可用于多模态成像引导的光热治疗。此外，我们的工作表明，金纳米诊疗剂可为癌症的诊断和治疗提供巨大的潜力。
