College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, China.
Poult Sci. 2021 Jan;100(1):348-356. doi: 10.1016/j.psj.2020.10.006. Epub 2020 Oct 13.
Heat stress (HS) in summer will seriously affect the health and performance of dairy cows. To alleviate the injury to dairy cows caused by HS, we added the rumen-bypassed tributyrin to the feed. We determined whether cows were in a heat-stressed environment by testing the temperature humidity index in the morning, at noon, and in the evening. The detection of anal temperature and respiratory frequency further proved the HS state of the dairy cows. The quantificational real time PCR results showed that tributyrin could significantly reduce the relative expression of tumor necrosis factor α, interleukin 1β, and Interleukin 6. Western blot results showed that tributyrin could alleviate the lymphocyte inflammatory response by inhibiting the mitogen-activated protein kinase and nuclear factor-кB signaling pathways. To further detect the effect of tributyrin on HS in dairy cows, routine biochemical and blood tests were carried out. The results showed that the contents of aspartate aminotransferase, total bilirubin, creatinine, albumin, and globulin were significantly reduced by tributyrin. The results showed that tributyrin could significantly alleviate the liver and kidney injury induced by heat stress in dairy cows. Moreover, tributyrin could also significantly reduce the numbers of intermediate cells and increase the level of hemoglobin. Tributyrin could also improve the performance of dairy cows. These results suggested that tributylglycerol may have a positive effect on breast health of dairy cows. In conclusion, these results indicated that tributyrin could relieve HS and increase the production performance of dairy cows by reducing the inflammatory responses of lymphocytes.
夏季热应激（HS）会严重影响奶牛的健康和生产性能。为了缓解 HS 对奶牛造成的伤害，我们在饲料中添加了瘤胃 bypass 丁酸甘油酯。我们通过测试奶牛在清晨、中午和傍晚的温度湿度指数来确定它们是否处于热应激环境中。肛温与呼吸频率的检测进一步证实了奶牛的 HS 状态。定量实时 PCR 结果表明，丁酸甘油酯可显著降低肿瘤坏死因子 α、白细胞介素 1β 和白细胞介素 6 的相对表达量。Western blot 结果表明，丁酸甘油酯通过抑制丝裂原活化蛋白激酶和核因子-кB 信号通路来减轻淋巴细胞的炎症反应。为了进一步检测丁酸甘油酯对奶牛 HS 的影响，我们进行了常规生化和血液检测。结果表明，丁酸甘油酯可显著降低天冬氨酸转氨酶、总胆红素、肌酐、白蛋白和球蛋白的含量。结果表明，丁酸甘油酯可显著减轻奶牛 HS 引起的肝肾功能损伤。此外，丁酸甘油酯还可以显著降低中间细胞数量并增加血红蛋白水平。丁酸甘油酯还可以提高奶牛的生产性能。这些结果表明，丁酸甘油酯可能对奶牛的乳房健康有积极影响。总之，这些结果表明，丁酸甘油酯通过降低淋巴细胞的炎症反应，缓解 HS 并提高奶牛的生产性能。
