Tian Yulan, Du Liping, Zhu Ping, Chen Yating, Chen Wei, Wu Chunsheng, Wang Ping
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, Xi'an, 710061, China.
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China; Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China, Xi'an, 710061, China; Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
Biosens Bioelectron. 2021 Mar 15;176:112899. doi: 10.1016/j.bios.2020.112899. Epub 2020 Dec 15.
Shellfish toxins, as one kind of marine toxin, have attracted worldwide attention due to their severe threat to food safety and human health. Therefore, it is highly essential and urgent to develop a low-cost and convenient method to detect these toxins. With the rapid advance in microfabrication processes, micro/nano biosensors provide novel approaches to address this issue. In addition to their features of low cost, portability, easy operation, high efficiency and high bioactivity, micro/nano biosensors have great potential to realize on-the-spot, rapid detection of shellfish toxins. This review focuses on the most recent advances in the development of micro/nano biosensors for shellfish toxin detection. These biosensors are mainly classified into five categories according to their transducer detection principles, which include optical devices, electrochemical sensors, electrochemiluminescence, field-effect transistors, and acoustic devices. Sensor strategies, toxin analytes, biosensitive elements, coupling methods and field detection performance are highlighted to discuss the applications of shellfish toxin detection. With advances in sensor technology, biomaterials, microfabrication and miniaturized electronics, micro/nano biosensors applied to in-field fast detection of shellfish toxins are expected to play a critical role in food safety, environmental monitoring, and foreign trade in the foreseeable future. Finally, the current challenges and future development trends of micro/nano biosensors for shellfish toxin detection are discussed.
贝类毒素作为一种海洋毒素，因其对食品安全和人类健康构成严重威胁而受到全球关注。因此，开发一种低成本、便捷的方法来检测这些毒素至关重要且迫在眉睫。随着微纳制造工艺的快速发展，微纳生物传感器为解决这一问题提供了新途径。除了具有低成本、便携、操作简便、高效和高生物活性等特点外，微纳生物传感器在实现贝类毒素的现场快速检测方面具有巨大潜力。本文综述了用于贝类毒素检测的微纳生物传感器的最新进展。这些生物传感器根据其换能器检测原理主要分为五类，包括光学器件、电化学传感器、电化学发光、场效应晶体管和声器件。重点介绍了传感器策略、毒素分析物、生物敏感元件、耦合方法和现场检测性能，以探讨贝类毒素检测的应用。随着传感器技术、生物材料、微纳制造和小型化电子学的发展，应用于贝类毒素现场快速检测的微纳生物传感器有望在可预见的未来在食品安全、环境监测和对外贸易中发挥关键作用。最后，讨论了用于贝类毒素检测的微纳生物传感器当前面临的挑战和未来发展趋势。
