Ripley-Petzoldt M L, Giebink G S, Juhn S K, Aeppli D, Tomasz A, Tuomanen E
Department of Pediatrics, School of Medicine, University of Minnesota, Minneapolis.
J Infect Dis. 1988 Feb;157(2):245-55. doi: 10.1093/infdis/157.2.245.
We studied the contribution of pneumococcal cell wall to the pathogenesis of otitis media in chinchillas after middle ear inoculation of killed, encapsulated type 7F Streptococcus pneumoniae; killed, unencapsulated R6 S. pneumoniae; and isolated R6 pneumococcal cell wall. Ears inoculated with encapsulated and unencapsulated pneumococci had significantly higher concentrations of polymorphonuclear and mononuclear leukocytes and lysozyme in middle ear fluid and developed more epithelial metaplasia and granulation tissue than did saline-inoculated ears. The mean concentration of lysozyme in middle ear fluid was higher in ears inoculated with killed, unencapsulated than encapsulated pneumococci. The middle ear mucoperiosteum of ears inoculated with pneumococcal cell wall showed significantly more polymorphonuclear leukocytes, epithelial metaplasia, subepithelial congestion, and granulation tissue than did control ears. Because nonviable, unencapsulated pneumococci and pneumococcal cell wall caused middle ear inflammation in the chinchilla model of otitis media, it is possible that cell envelope and cell wall components released during bacterial lysis may contribute to chronic otitis media with effusion in humans.
我们研究了在豚鼠中耳接种灭活的、有荚膜的7F型肺炎链球菌、灭活的、无荚膜的R6肺炎链球菌以及分离出的R6肺炎球菌细胞壁后，肺炎球菌细胞壁在中耳炎发病机制中的作用。接种有荚膜和无荚膜肺炎球菌的耳朵，其中耳液中的多形核白细胞和单核细胞浓度以及溶菌酶含量显著高于接种生理盐水的耳朵，并且比接种生理盐水的耳朵出现了更多的上皮化生和肉芽组织。接种灭活的无荚膜肺炎球菌的耳朵中耳液中溶菌酶的平均浓度高于接种有荚膜肺炎球菌的耳朵。接种肺炎球菌细胞壁的耳朵的中耳黏膜骨膜显示出比对照耳朵有更多的多形核白细胞、上皮化生、上皮下充血和肉芽组织。由于无活力的、无荚膜的肺炎球菌和肺炎球菌细胞壁在豚鼠中耳炎模型中引起了中耳炎症，因此细菌裂解过程中释放的细胞壁和细胞壁成分可能导致人类的慢性渗出性中耳炎。
