Taishan Institute for Hydrogen Biomedicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR China; Key Laboratory of Atherosclerosis in Universities of Shandong Province, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR China.
Taishan Institute for Hydrogen Biomedicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR China; Key Laboratory of Atherosclerosis in Universities of Shandong Province, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, PR China.
Life Sci. 2021 Feb 15;267:118945. doi: 10.1016/j.lfs.2020.118945. Epub 2020 Dec 29.
Oxidized phospholipids (OxPLs) are formed as a result of oxidative stress, which potentially mediate multiple pathological effects. We aimed to evaluate the effects of hydrogen (H) on OxPLs in vivo and the underlying mechanism.
Rats were randomly assigned to three groups: control group fed with a chow diet, model group fed with a high-fat diet, and H-treated group fed with a high-fat diet and treated by 4% H inhalation for ten weeks. OxPLs in liver and plasma were analyzed by liquid chromatography-mass spectrometry. High-density lipoprotein (HDL) was separated by ultracentrifugation. A proteomic analysis was performed to reveal the alternation of HDL protein composition and he antioxidant capacity of HDL was tested by low-density lipoprotein oxidation experiment. Furthermore, the activity or expression of HDL-associated enzymes were evaluated.
Inhalation of 4% H decreased the accumulation of OxPLs in rats. In vitro tests revealed that the different concentrations of H did not inhibit the formation of OxPLs mediated by non-enzymatic oxidation. H inhalation altered the components and enhanced the anti-oxidative capacity of HDL in rats fed with a high-fat diet. Further experiments showed that H significantly regulated the activity of lipoprotein-associated phospholipase A, paraoxonase-1, and the expression of lecithin:cholesterol acyltransferase.
Our findings revealed that H may reduce the OxPLs levels through its influence on HDL-associated enzymes that can act on OxPLs, suggesting that H can be used in alleviating diseases related to lipid peroxidation due to oxidative stress.
氧化磷脂（OxPLs）是氧化应激的结果，可能介导多种病理作用。我们旨在评估氢（H）对体内 OxPLs 的影响及其潜在机制。
大鼠随机分为三组：对照组给予常规饮食，模型组给予高脂饮食，H 治疗组给予高脂饮食并吸入 4%H 治疗 10 周。采用液相色谱-质谱法分析肝和血浆中的 OxPLs。通过超速离心分离高密度脂蛋白（HDL）。进行蛋白质组学分析以揭示 HDL 蛋白组成的改变，并通过低密度脂蛋白氧化实验测试 HDL 的抗氧化能力。此外，还评估了 HDL 相关酶的活性或表达。
吸入 4%H 可减少高脂饮食大鼠 OxPLs 的积累。体外试验表明，不同浓度的 H 不能抑制非酶氧化介导的 OxPLs 的形成。H 吸入改变了高脂饮食大鼠 HDL 的成分并增强了其抗氧化能力。进一步的实验表明，H 可显著调节脂蛋白相关磷脂酶 A、对氧磷酶-1 的活性和卵磷脂胆固醇酰基转移酶的表达。
我们的研究结果表明，H 可能通过影响可作用于 OxPLs 的 HDL 相关酶来降低 OxPLs 水平，提示 H 可用于缓解因氧化应激导致的脂质过氧化相关疾病。
