Federal University of Sergipe-UFS, Postgraduate Program in Materials Science and Engineering, São Cristóvão, Sergipe, Brazil; Federal University of Amapá-UNIFAP, Amazonian Materials Group, Physics Department, Macapá, Amapá, Brazil.
Federal University of Sergipe-UFS, Postgraduate Program in Materials Science and Engineering, São Cristóvão, Sergipe, Brazil.
Micron. 2021 Mar;142:102996. doi: 10.1016/j.micron.2020.102996. Epub 2020 Dec 16.
We performed qualitative and quantitative analysis of surfaces of kefir biofilms loaded with Amazon rainforest fruit extract. Scanning electron microscopy and atomic force microscopy were used to evaluate the micromorphology of the biofilms. The films surface displayed a lower density of microorganisms (∼ 0.061 microorganisms/μm) for the lowest concentration of fruit extract, however, a greater density (∼0.220 microorganisms/μm) was observed for the higher concentration. Height stereometric parameters revealed that the biofilms with the highest concentration presented the highest roughness. However, almost all the stereometric parameters related to texture showed no significant difference. Furthermore, the Hurst coefficients of the average power spectrum density were similar for all biofilms. Fractal parameters confirmed that higher concentrations of fruit extract induced a superior topographic irregularity. However, fractal lacunarity does not show any significant difference confirming the similarity of the microtextures. Moreover, fractal succolarity and surface entropy exhibited values that suggested ideal percolation and strong topographic uniformity, respectively, indicating that these films can uniformly adhere to other surfaces. Our results confirm that the stereometric and fractal parameters can be relevant for the surface characterization of microbial films, which can be of great importance to the biomedical field.
我们对加载有亚马逊雨林水果提取物的克菲尔生物膜的表面进行了定性和定量分析。扫描电子显微镜和原子力显微镜用于评估生物膜的微观形态。结果显示，对于最低浓度的水果提取物，薄膜表面的微生物密度较低（约 0.061 个/μm），而对于较高浓度的水果提取物，微生物密度较高（约 0.220 个/μm）。高度立体参数显示，浓度最高的生物膜具有最高的粗糙度。然而，几乎所有与纹理相关的立体参数都没有显著差异。此外，所有生物膜的平均功率谱密度的赫斯特系数都相似。分形参数证实，较高浓度的水果提取物会导致更优越的地形不规则性。然而，分形空隙度没有显示出任何显著差异，这证实了微观纹理的相似性。此外，分形似花度和表面熵呈现的值表明理想的渗流和较强的地形均匀性，这表明这些薄膜可以均匀地附着在其他表面上。我们的结果证实，立体和分形参数可用于微生物膜的表面特征化，这对于生物医学领域非常重要。
