Federal University of Amapá, Amazonian Materials Group, Physics Department, Amapá, Brazil; Federal University of Sergipe, Materials Engineering Department, Sergipe, Brazil.
Postgraduate Program in Tropical Medicine, State University of Amazonas, Manaus, Amazonas, Brazil.
Micron. 2020 Oct;137:102912. doi: 10.1016/j.micron.2020.102912. Epub 2020 Jun 18.
In this work, an advanced analysis of the 3D surface microtexture of the microbial films grown on Kefir loaded with Açaí extract was performed. Atomic force microscopy was used to characterize the 3D surface microtexture data in correlation with the stereometric analyses to allow a better understanding of the surface micromorphology consistent with ISO 25178-2: 2012. Two new parameters, fractal succolarity and fractal lacunarity, have been inserted for a quantitative approach to microtexture. The results revealed that the morphology was affected by the increase of the Açaí concentration in biofilms, as well as the fractal succolarity and lacunarity. Adhesive bacteria of the genus Lactobacillus were observed for the lowest concentrations of Açaí. Moreover, it was found that the surface of the biofilms has shown saturation when the concentration has changed from 4 to 6 % of Açaí. These results are of great interest in the characterization of surfaces with promising application like skin dressing.
本工作对负载巴西莓提取物的开菲尔中生长的微生物膜的 3D 表面微观结构进行了深入分析。原子力显微镜用于对 3D 表面微观结构数据进行特征化，并与立体分析相关联，以更好地理解符合 ISO 25178-2:2012 的表面微观形貌。为了进行定量分析，引入了两个新参数：分形填充度和分形空洞度。结果表明，形态受到生物膜中巴西莓浓度增加的影响，以及分形填充度和分形空洞度的影响。在巴西莓浓度最低的情况下，观察到了属于乳杆菌属的粘性细菌。此外，当巴西莓浓度从 4%变化到 6%时，发现生物膜的表面已经达到饱和。这些结果对于具有皮肤敷料等潜在应用的表面特征化具有重要意义。
