Postgraduate Program in Tropical Medicine, Fundação de Medicina Tropical, State University of Amazonas, Manaus69040-000, AM, Brazil.
Amazonian Materials Group, Physics Department, Federal University of Amapá-UNIFAP, Macapá, Amapá, Brazil.
Microsc Microanal. 2020 Oct;26(5):989-996. doi: 10.1017/S1431927620001798.
This work applies stereometric parameters and fractal theory to characterize the structural complexity of the 3D surface roughness of Anacardium occidentale L. leaf using atomic force microscopy (AFM) measurements. Surface roughness was studied by AFM in tapping mode, in air, on square areas of 6,400 and 10,000 μm2. The stereometric analyses using MountainsMap Premium and WSXM software provided detailed information on the 3D surface topography of the samples. These data showed that the morphology of the abaxial and adaxial side of the cashew leaf is different, which was also observed in relation to their microtextures. Fractal analysis showed that the adaxial and abaxial sides have strong microtexture homogeneity, but the adaxial side presented higher surface entropy. These results show that image processing associated with fractal theory can be an indispensable tool for identifying plant species by their leaves because this species has singularities on each side of the leaf.
本工作应用立体参数和分形理论，采用原子力显微镜（AFM）测量，对腰果叶的 3D 表面粗糙度的结构复杂性进行了表征。使用 AFM 在轻敲模式下，在空气中，对 6400 和 10000 μm2 的正方形区域进行了表面粗糙度研究。使用 MountainsMap Premium 和 WSXM 软件进行的立体分析提供了有关样品 3D 表面形貌的详细信息。这些数据表明，腰果叶的背面和正面的形态不同，这也与它们的微观结构有关。分形分析表明，正面和背面具有很强的微观纹理均匀性，但正面的表面熵更高。这些结果表明，与分形理论相关的图像处理可以成为通过叶片识别植物物种的不可或缺的工具，因为该物种在叶片的每一侧都具有奇异点。
