Sevastopol State University, Sevastopol, Russia.
Tomsk State University, Tomsk, Russia.
Sci Rep. 2023 Jul 5;13(1):10917. doi: 10.1038/s41598-023-33881-x.
Using AFM methods in air under normal conditions in a wide range of local force effects ([Formula: see text]< 40 μN) the relief, functional micromechanical properties (elasticity coefficient [Formula: see text], Young's modulus [Formula: see text], elastic [Formula: see text] and plastic [Formula: see text] deformations) and adhesive properties (work [Formula: see text] of adhesive forces [Formula: see text]) of the membranes of living adult cells of human buccal epithelium were studied in the presence of a protective layer < 100 nm of buffer solution that prevented the cells from drying. Almost all geometric and functional characteristics of the membrane in the local approximation at the micro- and nanolevels are affected by size effects and obey the laws of fractal geometry. The Brownian multifractal relief of the membrane is characterized by dimension [Formula: see text] < 2.56 and irregularities < 500 nm vertically and < 2 μm horizontally. Its response to elastic (≤ 6 nN), active (6-21 nN), or passive (> 21 nN) stimulation ([Formula: see text]) is a non-trivial selective process and exhibits a correspondingly elastic ([Formula: see text] 67.4 N/m), active ([Formula: see text] 80.2 N/m) and passive ([Formula: see text] 84.5 N/m) responses. [Formula: see text] and [Formula: see text] depend on [Formula: see text]. Having undergone slight plastic deformations [Formula: see text] < 300 nm, the membrane is capable of restoring its shape. We mapped ([Formula: see text], [Formula: see text] = 2.56; [Formula: see text], [Formula: see text] = 2.68; [Formula: see text], [Formula: see text] = 2.42, [Formula: see text] and [Formula: see text]) indicating its complex cavernous structure.
在正常条件下的空气中使用原子力显微镜方法（AFM），在[Formula: see text]（<40 μN）的局部力作用范围内，研究了人类口腔上皮活成年细胞的膜的缓解、功能微观机械性能（弹性系数[Formula: see text]、杨氏模量[Formula: see text]、弹性[Formula: see text]和塑性[Formula: see text]变形）和粘弹性（[Formula: see text]的粘性力[Formula: see text]的功）。在<100nm 的缓冲溶液保护层存在的情况下，研究了活成年细胞的膜的功能微观机械性能和粘弹性，该保护层防止细胞干燥。几乎所有的膜的微观和纳米水平的几何和功能特性都受到尺寸效应的影响，并服从分形几何的规律。膜的布朗多重分形起伏特征维度[Formula: see text] < 2.56，不规则度垂直方向<500nm，水平方向<2μm。它对弹性（≤6nN）、主动（6-21nN）或被动（>21nN）刺激[Formula: see text]的响应是一个非平凡的选择过程，并表现出相应的弹性（[Formula: see text]67.4N/m）、主动（[Formula: see text]80.2N/m）和被动（[Formula: see text]84.5N/m）响应。[Formula: see text]和[Formula: see text]取决于[Formula: see text]。膜经历轻微的塑性变形[Formula: see text] < 300nm 后，能够恢复其形状。我们绘制了膜的复杂洞穴结构的映射图（[Formula: see text]，[Formula: see text] = 2.56；[Formula: see text]，[Formula: see text] = 2.68；[Formula: see text]，[Formula: see text] = 2.42，[Formula: see text]和[Formula: see text]）。
