Département de biochimie, de microbiologie, et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec City, QC, Canada; Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Université Laval, Québec City, QC, Canada.
Département de biochimie, de microbiologie, et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec City, QC, Canada; Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Université Laval, Québec City, QC, Canada; Félix d'Hérelle Reference Center for Bacterial Viruses, Faculté de médecine dentaire, Université Laval, Québec City, QC, Canada.
Curr Opin Biotechnol. 2021 Apr;68:174-180. doi: 10.1016/j.copbio.2020.11.012. Epub 2020 Dec 23.
Antimicrobial resistance has spread quickly on a worldwide scale, reducing therapeutic options for bacterial infections. CRISPR-Cas is an adaptive immune system found in many prokaryotes that can be designed to target bacterial genomes, leading to cell death. Repurposing the CRISPR-Cas system as a therapeutic strategy offers an attractive way to overcome antimicrobial resistance. However, this strategy requires efficient vectors for the CRISPR-Cas system to reach the bacterial genomes. Engineered phages offer an attractive option as cargo delivery vectors. In this review, we discuss the production of phage-based vectors and the relevance of using repurposed CRISPR-Cas systems as antimicrobials. We also discuss recent progress in phage engineering that can potentially overcome the limitations and increase the efficiency of CRISPR-Cas delivery.
抗药性在全球范围内迅速传播，减少了治疗细菌感染的选择。CRISPR-Cas 是许多原核生物中发现的一种适应性免疫系统，可以被设计来靶向细菌基因组，导致细胞死亡。将 CRISPR-Cas 系统重新用于治疗策略提供了一种有吸引力的方法来克服抗药性。然而，这种策略需要有效的载体来使 CRISPR-Cas 系统到达细菌基因组。工程噬菌体作为货物递送载体提供了一种有吸引力的选择。在这篇综述中，我们讨论了基于噬菌体的载体的生产以及重新利用 CRISPR-Cas 系统作为抗菌剂的相关性。我们还讨论了噬菌体工程的最新进展，这些进展有可能克服限制并提高 CRISPR-Cas 传递的效率。
