Kongsoi Siriporn, Chumsing Suksun, Satorn Darunee, Noourai Panisa
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand.
Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand.
Vet World. 2020 Nov;13(11):2312-2318. doi: 10.14202/vetworld.2020.2312-2318. Epub 2020 Nov 3.
is an important foodborne pathogen and is recognized as a major public health issue. The emergence of multidrug-resistant (MDR) represents a major challenge for national public health authorities. We investigated the distribution of serovars and antimicrobial resistance of isolates from clinical swine samples stored at the Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University from 2016 to 2017.
Clinical samples were collected and subjected to standard microbiological techniques outlined in the Manual of Clinical Microbiology to identify serovars. Susceptibility to antimicrobials was tested by the Kirby-Bauer disk diffusion method using a panel of 14 antimicrobials.
A total of 144 isolates were identified and the dominant serovar was Choleraesuis (66.67%), followed by monophasic Typhimurium (18.75%), S. Typhimurium (9.03%), and Rissen (5.56%). The isolates displayed high resistance rates to ampicillin (AMP [100%]), amoxicillin (AX [100%]), tetracycline (TE [100%]), cefotaxime (CTX [89.58%]), ceftriaxone (CRO [87.50%]), chloramphenicol (C [82.64%]), gentamicin (CN [79.17%]), nalidixic acid (NA [72.92%]), and ceftazidime (CAZ [71.53%]). All isolates were MDR, with 29 distinct resistance patterns. The dominant MDR pattern among serovars Choleraesuis and Rissen exhibited resistance to 9 antimicrobials: (R7-14 AMP-AX-CAZ-CRO-CTX-NA-C-CN-TE). However, all tested isolates were susceptible to AX/clavulanic acid and fosfomycin.
High resistance levels to the third generation of cephalosporins such as CAZ, CRO, and CTX highlight the need for careful and reasonable usage of antimicrobials in animals and humans, especially for . Choleraesuis infections.
是一种重要的食源性病原体，被视为主要的公共卫生问题。多重耐药（MDR）的出现对国家公共卫生当局构成了重大挑战。我们调查了2016年至2017年存于 Kasetsart 大学兽医学院兽医诊断实验室的临床猪样本中分离株的血清型分布及抗菌药物耐药性。
收集临床样本，并按照《临床微生物学手册》中概述的标准微生物学技术来鉴定血清型。使用一组14种抗菌药物，通过 Kirby-Bauer 纸片扩散法检测对抗菌药物的敏感性。
共鉴定出144株分离株，优势血清型为猪霍乱沙门氏菌（66.67%），其次是单相鼠伤寒沙门氏菌（18.75%）、鼠伤寒沙门氏菌（9.03%）和里森沙门氏菌（5.56%）。这些分离株对氨苄西林（AMP [100%]）、阿莫西林（AX [100%]）、四环素（TE [100%]）、头孢噻肟（CTX [89.58%]）、头孢曲松（CRO [87.50%]）、氯霉素（C [82.64%]）、庆大霉素（CN [79.17%]）、萘啶酸（NA [72.92%]）和头孢他啶（CAZ [71.53%]）显示出高耐药率。所有分离株均为多重耐药，有29种不同的耐药模式。猪霍乱沙门氏菌和里森沙门氏菌血清型中的优势多重耐药模式表现出对9种抗菌药物的耐药性：（R7 - 14 AMP - AX - CAZ - CRO - CTX - NA - C - CN - TE）。然而，所有测试分离株对 AX/克拉维酸和磷霉素敏感。
对第三代头孢菌素如 CAZ、CRO 和 CTX 的高耐药水平凸显了在动物和人类中谨慎合理使用抗菌药物的必要性，尤其是对于猪霍乱沙门氏菌感染。
