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盘基网柄菌中一个受发育调控的膜蛋白基因也受热激和冷激诱导。

A developmentally regulated membrane protein gene in Dictyostelium discoideum is also induced by heat shock and cold shock.

作者信息

Maniak M, Nellen W

机构信息

Max-Planck-Institut fuer Biochemie, Abteilung Zellbiologie, Martinsried, Federal Republic of Germany.

出版信息

Mol Cell Biol. 1988 Jan;8(1):153-9. doi: 10.1128/mcb.8.1.153-159.1988.

DOI:10.1128/mcb.8.1.153-159.1988
PMID:3336356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC443571/
Abstract

We have analyzed the expression of the Dictyostelium gene P8A7 which had been isolated as a cDNA clone from an early developmentally regulated gene. The single genomic copy generated two mRNAs which were subject to different control mechanisms: while one mRNA (P8A7S) was regulated like the cell-type-nonspecific late genes, the other one (P8A7L) was induced during development, when cells were allowed to attach to a substrate, and when cells were subjected to stress, such as heat shock and cadmium. Interestingly the same induction was also observed with cold shock. RNA processing was inhibited by heat and cold shock, leading to nuclear accumulation of a precursor. The translated region of the cDNA was common to both mRNAs and encoded an unusually hydrophobic peptide with the characteristics of a membrane protein.

摘要

我们分析了盘基网柄菌基因P8A7的表达情况，该基因是从一个早期发育调控基因中分离得到的cDNA克隆。单个基因组拷贝产生了两种mRNA，它们受到不同的调控机制：一种mRNA（P8A7S）的调控方式类似于细胞类型非特异性晚期基因，而另一种（P8A7L）在发育过程中被诱导，当细胞附着于底物时，以及当细胞受到热休克和镉等应激时。有趣的是，冷休克也观察到相同的诱导现象。热休克和冷休克均抑制RNA加工，导致前体在细胞核中积累。cDNA的编码区在两种mRNA中是相同的，编码一种具有膜蛋白特征的异常疏水肽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/5e4f9a601103/molcellb00061-0179-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/07fb572bcfac/molcellb00061-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/398114d10514/molcellb00061-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/23d110599a07/molcellb00061-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/fb3d8abc8361/molcellb00061-0178-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/3811521ae812/molcellb00061-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/5e4f9a601103/molcellb00061-0179-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/07fb572bcfac/molcellb00061-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/398114d10514/molcellb00061-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/23d110599a07/molcellb00061-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/fb3d8abc8361/molcellb00061-0178-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/3811521ae812/molcellb00061-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8822/443571/5e4f9a601103/molcellb00061-0179-b.jpg

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本文引用的文献

1
Identification of a DNA sequence element required for efficient expression of a developmentally regulated and cAMP-inducible gene of Dictyostelium discoideum.鉴定一个 DNA 序列元件，该元件是秀丽隐杆线虫中发育调控和 cAMP 诱导基因高效表达所必需的。
EMBO J. 1987 Jan;6(1):195-200. doi: 10.1002/j.1460-2075.1987.tb04738.x.
2
Complete sequence and transcript regulation of a cell adhesion protein from aggregating Dictyostelium cells.完整的细胞黏附蛋白序列及其在聚集型 Dictyostelium 细胞中的转录调控。
EMBO J. 1986 Jul;5(7):1473-6. doi: 10.1002/j.1460-2075.1986.tb04384.x.
3
The 'light' and 'medium' subunits of the photosynthetic reaction centre from Rhodopseudomonas viridis: isolation of the genes, nucleotide and amino acid sequence.
在抑制芽孢形成温度下非生长型巨大芽孢杆菌的细胞活力和蛋白质周转
Folia Microbiol (Praha). 1999;44(3):271-5. doi: 10.1007/BF02818546.
4
Sequence and molecular characterization of a DNA region encoding a small heat shock protein of Clostridium acetobutylicum.丙酮丁醇梭菌一种小热休克蛋白编码DNA区域的序列及分子特征分析
J Bacteriol. 1993 Jun;175(11):3394-400. doi: 10.1128/jb.175.11.3394-3400.1993.
5
Poly(A) tail length of a heat shock protein RNA is increased by severe heat stress, but intron splicing is unaffected.热休克蛋白RNA的聚腺苷酸尾长度在严重热应激下会增加，但内含子剪接不受影响。
Mol Gen Genet. 1993 Jun;239(3):323-33. doi: 10.1007/BF00276930.
6
Heat shock affects 5' splice site selection, cleavage and ligation of CAD pre-mRNA in hamster cells, but not its packaging in InRNP particles.热休克影响仓鼠细胞中CAD前体mRNA的5'剪接位点选择、切割和连接，但不影响其在InRNP颗粒中的包装。
Nucleic Acids Res. 1994 Aug 11;22(15):3084-91. doi: 10.1093/nar/22.15.3084.
7
Codon preference in Dictyostelium discoideum.盘基网柄菌中的密码子偏好性。
Nucleic Acids Res. 1988 Jul 25;16(14A):6617-35. doi: 10.1093/nar/16.14.6617.
8
The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs.盘基网柄菌的环核苷酸磷酸二酯酶基因利用交替启动子和剪接来合成多种mRNA。
Mol Cell Biol. 1989 Sep;9(9):3938-50. doi: 10.1128/mcb.9.9.3938-3950.1989.
9
Expression and organization of BP74, a cyclic AMP-regulated gene expressed during Dictyostelium discoideum development.BP74的表达与组织，BP74是一种在盘基网柄菌发育过程中表达的环磷酸腺苷调节基因。
Mol Cell Biol. 1989 Oct;9(10):4170-8. doi: 10.1128/mcb.9.10.4170-4178.1989.
10
Two separable promoters control different aspects of expression of a Dictyostelium gene.两个可分离的启动子控制盘基网柄菌属基因表达的不同方面。
Nucleic Acids Res. 1990 Jun 11;18(11):3211-7. doi: 10.1093/nar/18.11.3211.
绿硫红假单胞菌光合反应中心的“轻”和“中”亚基：基因的分离、核苷酸和氨基酸序列
EMBO J. 1986 Jun;5(6):1149-58. doi: 10.1002/j.1460-2075.1986.tb04340.x.
4
Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.盘基网柄菌中的序列组织：蛋白质编码基因5'端的独特结构
Nucleic Acids Res. 1983 Jan 25;11(2):541-52. doi: 10.1093/nar/11.2.541.
5
Heat shock response of Dictyostelium.盘基网柄菌的热休克反应
Dev Biol. 1980 Oct;79(2):399-408. doi: 10.1016/0012-1606(80)90125-6.
6
Mutants of Dictyostelium discoideum blocked in expression of all members of the developmentally regulated discoidin multigene family.盘基网柄菌的突变体在发育调控的盘状菌素多基因家族所有成员的表达中受阻。
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7
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Cell. 1983 Apr;32(4):1139-48. doi: 10.1016/0092-8674(83)90297-0.
8
Induction and modulation of cell-type-specific gene expression in Dictyostelium.盘基网柄菌中细胞类型特异性基因表达的诱导与调控
Cell. 1983 Mar;32(3):763-71. doi: 10.1016/0092-8674(83)90062-4.
9
A change in the rate of transcription of a eukaryotic gene in response to cyclic AMP.真核基因转录速率响应环磷酸腺苷（cAMP）的变化。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7171-5. doi: 10.1073/pnas.77.12.7171.
10
An unusual transposon encoding heat shock inducible and developmentally regulated transcripts in Dictyostelium.一种不寻常的转座子，其编码在盘基网柄菌中热休克诱导且受发育调控的转录本。
Cell. 1983 Nov;35(1):243-51. doi: 10.1016/0092-8674(83)90227-1.