Alexander S, Shinnick T M, Lerner R A
Cell. 1983 Sep;34(2):467-75. doi: 10.1016/0092-8674(83)90380-x.
Mutant strains of D. discoideum are described that can complete morphogenesis and cytodifferentiation but which express vastly reduced levels of the galactose-binding lectins discoidin I and II (less than 1% and 1%-2% respectively) compared to the wild-type control. Mutant cells proceeding through development lack lectin activity, lectin protein, and specific lectin mRNA. In contrast, the genes encoding these proteins are present in their wild-type configurations in the genome. Since these proteins are encoded by four to five discrete genes, the mutations in these strains are most likely in genes involved in the regulation of the expression of members of this multigene family. The results also indicate that the discoidin lectins may not be required for fruiting body construction in this organism. Finally, coupled with the recent ability to transform D. discoideum, these mutants open the way to identification and isolation of regulatory genes and their products.
描述了盘基网柄菌的突变菌株，这些菌株能够完成形态发生和细胞分化，但与野生型对照相比，其半乳糖结合凝集素盘基网柄菌素I和II的表达水平大幅降低（分别低于1%和1%-2%）。进行发育的突变细胞缺乏凝集素活性、凝集素蛋白和特定的凝集素mRNA。相反，编码这些蛋白质的基因以其野生型构型存在于基因组中。由于这些蛋白质由四到五个离散基因编码，这些菌株中的突变很可能发生在参与调控这个多基因家族成员表达的基因中。结果还表明，盘基网柄菌素凝集素可能不是该生物体子实体构建所必需的。最后，结合最近转化盘基网柄菌的能力，这些突变体为鉴定和分离调控基因及其产物开辟了道路。
