Cho Myong-Suk, Kim Yongsung, Kim Seung-Chul, Park Jongsun
Department of Biological Sciences, Sungkyunkwan University, Suwon, Republic of Korea.
InfoBoss Co., Ltd., Seoul, Republic of Korea.
Mitochondrial DNA B Resour. 2019 Jul 12;4(2):2424-2425. doi: 10.1080/23802359.2019.1598802.
Maxim. is one of the most important pear species cultivated in Asia, grown in northern China, far-east Russia, Korea, and Japan. Here we completed the chloroplast genome of wild collected in Bonghwa-gun, Korea, which was 159,986 bp in length consisting of four subregions: 87,947 bp of large single copy (LSC) and 19,255 bp of small single copy (SSC) regions are separated by 26,392 bp of inverted repeat (IR) regions. The genome contained a total of 130 genes including 85 protein-coding genes, eight rRNAs, and 37 tRNAs. The overall GC content was 36.5% and those in the LSC, SSC, and IR regions were 34.2%, 30.4%, and 42.6%, respectively. Phylogenetic analysis of 14 chloroplast genomes provided the diverse genetic background for wild populations in Korea by confirming that wild sequenced in this study contained type plastome. It revealed substantial sequence variations up to 121 single nucleotide polymorphisms and 781 insertions and deletions against another wild accession of ( type) collected in Mt. Hambaek, Korea.
秋子梨是亚洲栽培的最重要的梨品种之一，在中国北方、俄罗斯远东地区、韩国和日本均有种植。在此，我们完成了从韩国奉化郡采集的野生秋子梨叶绿体基因组测序，其长度为159,986 bp，由四个亚区组成：87,947 bp的大单拷贝（LSC）区和19,255 bp的小单拷贝（SSC）区被26,392 bp的反向重复（IR）区隔开。该基因组共包含130个基因，其中包括85个蛋白质编码基因、8个rRNA和37个tRNA。总体GC含量为36.5%，LSC、SSC和IR区的GC含量分别为34.2%、30.4%和42.6%。对14个叶绿体基因组的系统发育分析证实，本研究测序的野生秋子梨包含G型质体基因组，为韩国野生秋子梨种群提供了多样的遗传背景。与在韩国汉白山脉采集的另一个野生秋子梨（H型）相比，它显示出多达121个单核苷酸多态性和781个插入缺失的大量序列变异。
