Chung Ho Yong, Lee Tae-Ho, Kim Yoon-Kyeong, Kim Jung Sun
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju, South Korea.
Pear Research Institute, National Institute of Horticultural & Herbal Science, RDA, Naju, South Korea.
Mitochondrial DNA B Resour. 2017 May 30;2(1):325-326. doi: 10.1080/23802359.2017.1331328.
The complete chloroplast genome of Wonwhang (BioSample SAMN05196235), , was assembled and analyzed by assembly using whole-genome sequencing data. The accession NC_015996 was used as a reference sequence in this study. The total chloroplast genome size of the Wonwhang was 159,922 bp in length, including a pair of inverted repeat regions (IRs) of 26,392 bp that are separated by a large single-copy region of 72,023 bp and a small single-copy region of 19,235 bp. A total of 132 genes, including 93 protein-coding genes, 31 tRNA genes and eight rRNA genes, were predicted from the chloroplast genomes. Among them, 18 genes occur in IRs, containing nine protein-coding genes, five tRNA genes and four rRNA genes. The GC content of Wonwhang chloroplast genome is 36.6%. The phylogenetic analysis with nine Rosids species and three other species revealed that Wonwhang was clustered with genus.
使用全基因组测序数据通过组装对圆黄梨（生物样本SAMN05196235）的完整叶绿体基因组进行了组装和分析。本研究中使用登录号为NC_015996的序列作为参考序列。圆黄梨的叶绿体基因组全长159,922 bp，包括一对26,392 bp的反向重复区域（IRs），它们被一个72,023 bp的大单拷贝区域和一个19,235 bp的小单拷贝区域隔开。从叶绿体基因组中预测出总共132个基因，包括93个蛋白质编码基因、31个tRNA基因和8个rRNA基因。其中，18个基因位于IRs中，包括9个蛋白质编码基因、5个tRNA基因和4个rRNA基因。圆黄梨叶绿体基因组的GC含量为36.6%。对9种蔷薇类物种和其他3个物种的系统发育分析表明，圆黄梨与[具体属名未给出]属聚类在一起。
