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‘云红梨1号’叶绿体全基因组特征分析

Characterization of the complete chloroplast genome of 'Yunhongli No. 1'.

作者信息

Cheng Longjie, Wang Yuying, Yang Jin, He Yingyun, Li Guochang, Ma Weirong, Huang Xinglong, Su Jun

机构信息

Institute of Horticulture, Yunnan Academy of Agricultural Sciences, Kunming, PR China.

College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, PR China.

出版信息

Mitochondrial DNA B Resour. 2020 Jul 30;5(3):3100-3102. doi: 10.1080/23802359.2020.1800429.

DOI:10.1080/23802359.2020.1800429
PMID:33458073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7781984/
Abstract

'Yunhongli No. 1' is a rare and well-colored red pear () germplasm resource, and is popular in the market due to its bright red color and high quality. This study used Illumina high-throughput sequencing technologies for red pear 'Yunhongli No. 1' of chloroplast genome sequencing and analysis. The genome features of 'Yunhongli No. 1' and the phylogenetic relationships were reported and established. The complete chloroplast genome is 160,113 bp in length, consisting of a pair of inverse duplication regions 26,386 bp, a large single-copy region 88,052 bp, and a small single-copy region 19,214 bp. The entire genome contains 80 messenger RNA (mRNA) genes, 30 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes. The phylogenetic tree of 15 species revealed red pear 'Yunhongli No. 1' is more closely related to × 'Greensis.'

摘要

“云红梨1号”是一种珍稀且色泽优良的红梨()种质资源,因其鲜红色泽和高品质而在市场上颇受欢迎。本研究采用Illumina高通量测序技术对红梨“云红梨1号”进行叶绿体基因组测序与分析。报道并建立了“云红梨1号”的基因组特征及系统发育关系。完整的叶绿体基因组长度为160,113 bp,由一对26,386 bp的反向重复区域、一个88,052 bp的大单拷贝区域和一个19,214 bp的小单拷贝区域组成。整个基因组包含80个信使核糖核酸(mRNA)基因、30个转运核糖核酸(tRNA)基因和4个核糖体核糖核酸(rRNA)基因。15个物种的系统发育树显示红梨“云红梨1号”与דGreensis”的亲缘关系更近。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f5d/7781984/25a455ecfc79/TMDN_A_1800429_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f5d/7781984/25a455ecfc79/TMDN_A_1800429_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f5d/7781984/25a455ecfc79/TMDN_A_1800429_F0001_B.jpg

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本文引用的文献

1
GeSeq - versatile and accurate annotation of organelle genomes.GeSeq - 细胞器基因组的多功能和准确注释。
Nucleic Acids Res. 2017 Jul 3;45(W1):W6-W11. doi: 10.1093/nar/gkx391.
2
NOVOPlasty: de novo assembly of organelle genomes from whole genome data.NOVOPlasty:从头组装细胞器基因组的全基因组数据。
Nucleic Acids Res. 2017 Feb 28;45(4):e18. doi: 10.1093/nar/gkw955.
3
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.MEGA7:适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.