Zhi-Min Jin, Ke-Song Qin, Bo-Qi Li, Jun-Sheng Zhang, He Cai, Jin-Xun Li, Zhu Wang, Zhu Liu
College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Aug 29;4(2):2791-2793. doi: 10.1080/23802359.2019.1659118.
The complete mitogenome sequence of was determined using long PCR. The genome was 16,969 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (32.5%), C (22.3%), T (31.9%), and G (13.3%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five species formed a monophyletic group with the high bootstrap value (100%) in all examinations. This study verifies the evolutionary status of in Soricidae at the molecular level. The mitochondrial genome would be a significant supplement for the genetic background.
使用长PCR技术测定了[物种名称]的完整线粒体基因组序列。该基因组长度为16,969 bp，包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因、1个轻链复制起点和1个控制区。重链的总体碱基组成为A（32.5%）、C（22.3%）、T（31.9%）和G（13.3%）。碱基组成明显呈现A-T偏斜，在控制区和蛋白质编码基因中最为明显。基于最大简约法（MP）、最大似然法（ML）、邻接法（NJ）和贝叶斯分析的线粒体基因组分析产生了相同的系统发育树。在所有检测中，这五个[物种名称]物种形成了一个具有高自展值（100%）的单系群。本研究在分子水平上验证了[物种名称]在鼩鼱科中的进化地位。线粒体基因组将为[物种名称]的遗传背景提供重要补充。
