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中国小竹鼠线粒体全基因组测序、分析及其系统发育分析

Sequencing and analysis of the complete mitochondrial genome of the lesser bandicoot rat () from China and its phylogenetic analysis.

作者信息

Zhu Liu, Qing-Qing Wang, Xue-Ting Jiang, Wen-Jing Jiang, Xin-Xu Zhao, Qian-Qian Yao, Jun-Sheng Zhang, Zhong-Wan Piao

机构信息

College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China.

出版信息

Mitochondrial DNA B Resour. 2021 Jun 21;6(7):2063-2065. doi: 10.1080/23802359.2021.1942273.

Abstract

The complete mitogenome sequence of the lesser bandicoot rat ( Gray and Hardwicke, 1833) was determined using long PCR. The genome was 16,327 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (34.2%), C (24.9%), T (28.5%) and G (12.4%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of in Muridae at the molecular level. The mitochondrial genome would be a significant supplement for the genetic background. The two species formed a monophyletic group with the high bootstrap value (100%) in all examinations.

摘要

采用长链PCR技术测定了小板齿鼠(Gray和Hardwicke,1833年)的完整线粒体基因组序列。该基因组长度为16327 bp,包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因、1个轻链复制起点和1个控制区。重链的总体碱基组成为A(34.2%)、C(24.9%)、T(28.5%)和G(12.4%)。碱基组成呈现明显的A-T偏斜,在控制区和蛋白质编码基因中最为明显。基于MP、ML、NJ和贝叶斯分析的线粒体基因组分析产生了相同的系统发育树。本研究在分子水平上验证了小板齿鼠在鼠科中的进化地位。线粒体基因组将为小板齿鼠的遗传背景提供重要补充。在所有检测中,这两个小板齿鼠物种形成了一个具有高自展值(100%)的单系类群。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f15/8218843/a70842dad309/TMDN_A_1942273_F0001_B.jpg

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