Jin Zhi-Min, Zhu Liu, Ma Jian-Zhang
College of Wildlife Resources, Northeast Forestry University, Harbin, P.R. China.
College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China.
Mitochondrial DNA B Resour. 2017 Jul 31;2(2):486-488. doi: 10.1080/23802359.2017.1361354.
The complete mitogenome sequence of the masked shrew () was determined using long PCR. The genome was 17,096 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (32.9%), C (24.5%), T (29.3%), and G (13.3%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of the masked shrew. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five species formed a monophyletic group with the high bootstrap value (100%) in all examinations.
利用长片段PCR技术测定了北美短尾鼩（）的完整线粒体基因组序列。该基因组长度为17,096 bp，包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因、1个轻链复制起点和1个控制区。重链的总体碱基组成为A（32.9%）、C（24.5%）、T（29.3%）和G（13.3%）。碱基组成明显呈现A-T偏斜，在控制区和蛋白质编码基因中最为明显。在北美短尾鼩的线粒体基因组控制区定义了延伸终止相关序列结构域、中央保守结构域和保守序列模块结构域。基于最大简约法（MP）、最大似然法（ML）、邻接法（NJ）和贝叶斯分析的线粒体基因组分析产生了相同的系统发育树。在所有检测中，这五个物种形成了一个具有高自展值（100%）的单系类群。
